Addgene

We narrowed to 876 results for: GGCT;

Showing: 461 - 480 of 876 results

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hU6-sgNT1 (opti)

Plasmid

#177237

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses non-targeting gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgNT1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgNeo1 (opti)

Plasmid

#177240

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses neomycin gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgNeo1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpb_v2 (opti)

Plasmid

#177248

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpb gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpb_2nd

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA

Plasmid

#178099

Purpose

Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G3 + MTOR nick intron-45 sgRNAs

Use

CRISPR; Prime editing

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G8_MTOR_Nick_Intron-45_Dual_sgRNA

Plasmid

#178106

Purpose

Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G8 + MTOR nick intron-45 sgRNAs

Use

CRISPR; Prime editing

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Grin1 gRNA#3

Plasmid

#169791

Purpose

Cas9-mediated knockout of Grin1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Grin1 (Grin1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-mHes1-L

Plasmid

#122309

Purpose

Expresses sgRNA targeting mouse Hes1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Hes1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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DYNLT3 B3.5 gRNA

Plasmid

#90670

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYNLT3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DYNLT3 (Guide Designation B3.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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Pmyo-3::hpo-30 gDNA (pBAB204)

Plasmid

#117385

Purpose

Allows for body-wall muscle expression of HPO-30

Depositor

Kavita Babu

Article

Sharma et al J Neurosci. 2018 Aug 8;38(32):7072-7087.

Insert

genomic DNA of hpo-30 (833 bp)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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RACGAP1 E7.4 gRNA

Plasmid

#90864

Purpose

3rd generation lentiviral gRNA plasmid targeting human RACGAP1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RACGAP1 (Guide Designation E7.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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PTTG1 A10.4 gRNA

Plasmid

#90863

Purpose

3rd generation lentiviral gRNA plasmid targeting human PTTG1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PTTG1 (Guide Designation A10.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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PCNT E1.4 gRNA

Plasmid

#90830

Purpose

3rd generation lentiviral gRNA plasmid targeting human PCNT

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PCNT (Guide Designation E1.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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NCAPG E9.2 gRNA

Plasmid

#90780

Purpose

3rd generation lentiviral gRNA plasmid targeting human NCAPG

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NCAPG (Guide Designation E9.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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NCAPG E10.2 gRNA

Plasmid

#90781

Purpose

3rd generation lentiviral gRNA plasmid targeting human NCAPG

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NCAPG (Guide Designation E10.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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AHCTF1 A7.2 gRNA

Plasmid

#90526

Purpose

3rd generation lentiviral gRNA plasmid targeting human AHCTF1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

AHCTF1 (Guide Designation A7.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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RRM2 E4.3 gRNA

Plasmid

#90883

Purpose

3rd generation lentiviral gRNA plasmid targeting human RRM2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RRM2 (Guide Designation E4.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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MAPRE1 F10.3 gRNA

Plasmid

#90747

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPRE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAPRE1 (Guide Designation F10.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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CDCA8 B5.6 gRNA

Plasmid

#90602

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDCA8

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDCA8 (Guide Designation B5.6)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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CENPW H4.3 gRNA

Plasmid

#90621

Purpose

3rd generation lentiviral gRNA plasmid targeting human CENPW

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CENPW (Guide Designation H4.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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GMNN D2.2 gRNA

Plasmid

#90697

Purpose

3rd generation lentiviral gRNA plasmid targeting human GMNN

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

GMNN (Guide Designation D2.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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