Addgene

We narrowed to 15,358 results for: LEA

Showing: 461 - 480 of 15358 results

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pRab-3::Cerulean-Venus::Lgg-1

Plasmid

#68045

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the neurons, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pRab-3::Cerulean-Venus::Lgg-1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST p38KTRmCerulean3

Plasmid

#59155

Purpose

Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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nuclear EKAR (EGFP-mRFP)

Plasmid

#18682

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Use

Tags

EGFP and mRFP

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Hygro DEST ERKKTRmCerulean3

Plasmid

#90229

Purpose

Lentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

ERK Kinase Translocation Reporter

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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mRNA-CleanCap entry vector

Plasmid

#188249

Purpose

Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTRmCerulean3

Plasmid

#90232

Purpose

Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ash-mCerulean

Plasmid

#69579

Purpose

Expresses p53 tagged with mCerulean

Depositor

Galit Lahav

Article

Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

Insert

p53 (TP53 Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Mutation

7 silent mutations in p53 DNA to prevent targetin…

Promoter

EF1alpha

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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His6-TEV-mCerulean3-pBluescript

Plasmid

#166757

Purpose

For purification of mCerulean3 fluorophore

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

mCerulean3

Use

Tags

6x Histidine tag, and TEV protease site

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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cytoplasmic EKAR (Cerulean-Venus)

Plasmid

#18679

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Use

Tags

monomeric Cerulean and monomeric Venus

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pOpen-Exonuclease III E. coli

Plasmid

#165571

Purpose

Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA.

Depositor

FreeGenes Project, Drew Endy, Jennifer Molloy

Insert

Exonuclease III E. coli

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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hSyn-DIO-somBiPOLES-mCerulean

Plasmid

#154951

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Has Service

AAV Retrograde, AAV5, and AAV9

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2-Venus

Plasmid

#138196

Purpose

FRET-based biosensor for monitoring Epac2 activation.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2-Venus (Rapgef4 Mouse, Synthetic)

Use

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp PHAkt Cerulean

Plasmid

#50837

Purpose

Fluorescent reporter for membrane PIP3 levels

Depositor

Wendell Lim

Article

Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

Insert

PHAkt (AKT1 )

Use

Lentiviral and Synthetic Biology

Tags

Cerulean

Expression

Mutation

Promoter

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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765 pME nls mCerulean

Plasmid

#195958

Purpose

Gateway pME fluorophore

Depositor

Christian Mosimann, Kristen Kwan

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mCerulean nuclear

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Venus/Cerulean-Synaptopodin-A in pcDNA6

Plasmid

#190798

Purpose

Imaging of synaptopodin in live cells

Depositor

Vivian Tang

Article

Morris et al J Cell Biol. 2022 May 2;221(5). pii: 213

Insert

Synaptopodin-A (SYNPO Human, Synthetic)

Use

Tags

Cerulean, His, Venus, and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJP-CoV2leader-nLuc-TSS

Plasmid

#175430

Purpose

Expresses nLuc with 5' leader of CoV2 in mammalian cells.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nLUC plus 5' CoV2 leader

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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769 pME mCerulean-CAAX

Plasmid

#195957

Purpose

Gateway pME fluorophore

Depositor

Christian Mosimann, Kristen Kwan

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mCerulean-CAAX (membrane)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Ef1a-DIO-BiPOLES-mCerulean

Plasmid

#154949

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1-alpha

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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E407V Nuclear Myosin I YFP

Plasmid

#60242

Purpose

Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

Depositor

Primal de Lanerolle

Article

Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

Insert

Nuclear Myosin 1 (Myo1c Mouse)

Use

Tags

EYFP and NLS

Expression

Mammalian

Mutation

E407V

Promoter

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
Cerulean-tagged Apollo-NADP+

Plasmid

#71800

Purpose

Expresses Cerulean-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

Depositor

Jonathan Rocheleau

Article

Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

Insert

Apollo-NADP+

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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