We narrowed to 12,237 results for: LEA

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pX330-EN1201

Plasmid

#92144

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

Depositor

Benoit Bruneau

Publication

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus

Expression

Mammalian

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pBAD24-sfGFPx1

Plasmid

#51558

Purpose

Superfolder GFP ORF cloned into pBAD24 for expression in E. coli

Depositor

Sankar Adhya, Francisco Malagon

Publication

Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

Insert

superfolder GFP

Expression

Bacterial

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3.1+ hnRNPK myc HIS

Plasmid

#71662

Purpose

Mammalian expression of hnRNPK

Depositor

Gideon Dreyfuss

Insert

hnRNPK (HNRNPK Human)

Tags

HIS and myc

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMX/cyclinB1-GFP (27)

Plasmid

#26061

Depositor

Jonathon Pines

Publication

Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

Insert

Cyclin B1 (CCNB1 Human)

Tags

GFP

Expression

Mammalian

Available since

Aug. 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3-N-Flag-Caspase-1

Plasmid

#75128

Purpose

Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

Depositor

Bruce Beutler

Publication

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

caspase 1 (Casp1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

P341S in Caspase-1 (please see depositor comments…

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pcDNA3.1-HA-NONO

Plasmid

#127655

Purpose

Over-express HA-tagged human NONO

Depositor

Nicolas Manel

Publication

Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

Insert

NONO (NONO Human)

Tags

HA

Expression

Mammalian

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Hnf4a-IRES-GFP

Plasmid

#33006

Depositor

Atsushi Suzuki

Publication

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

hepatic nuclear factor 4 alpha (Hnf4a Mouse)

Use

Retroviral

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pExodus CMV.Trex2

Plasmid

#40210

Purpose

Mammalian expression of codon-optimized Trex2.

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

Insert

Three prime repair exonuclease 2 (Trex2 Mouse)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

Plasmid

#122151

Purpose

Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

dCas9 fused to SunTag(24x)

Use

Lentiviral

Tags

2xNLS-P2A-BFP and NLS

Expression

Mammalian

Promoter

SFFV

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Nurr1

Plasmid

#35000

Depositor

Malin Parmar

Publication

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Nuclear receptor subfamily 4 group A member 2 (Nr4a2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pGL3-p300 reporter

Plasmid

#11726

Depositor

Eileen Adamson

Publication

Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

Insert

p300 promoter and 5' UTR (EP300 )

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 TFAM-mScarlet

Plasmid

#129573

Purpose

Expression of mScarlet fused TFAM

Depositor

Stephen Tait

Publication

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

TFAM (TFAM Human)

Expression

Mammalian

Mutation

None

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3 Ash2L full

Plasmid

#15548

Depositor

Kai Ge

Publication

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Ash2L (ASH2L Human)

Expression

Mammalian

Mutation

R622H (Please see depositor comments below)

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

Plasmid

#60815

Purpose

dCas9 with single cysteine residue (M1C) for site-specific labelling

Depositor

Jennifer Doudna

Publication

O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

Insert

dCas9 M1C D10A C80S H840A C574S

Tags

His10 and MBP

Expression

Bacterial

Mutation

M1C D10A C80S H840A C574S

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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mCherry-NbALFA

Plasmid

#159987

Purpose

For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)

Depositor

Motoyuki Hattori

Publication

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

mCherry-NbALFA

Tags

8histidine-HRV3C protease cleavage site

Expression

Bacterial

Mutation

3GS linker between mCherry and NbALFA

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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LBR pEGFP-N2 (646)

Plasmid

#61996

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Publication

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

LBR (LBR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMV-PV-NLS-GFP

Plasmid

#17300

Depositor

Anton Bennett

Publication

Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

Insert

PV-GFP-NLS (Pvalb Rat)

Tags

Myc and NLS

Expression

Mammalian

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-N-Flag-ASC1

Plasmid

#75134

Purpose

Expresses N-terminal Flag-tagged ASC1 in mammalian cells

Depositor

Bruce Beutler

Publication

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

PYD and CARD domain containing (Pycard Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

F23S (please see depositor comments below)

Promoter

CMV

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pET-29b(+)_NSP4ss_SerM_NucA-His

Plasmid

#218190

Purpose

Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

Depositor

Florence Pojer

Publication

Rieubon et al protocols.io 2026. doi: https://dx.doi.o

Insert

nucA

Tags

10xHis and NSP4 signal secretion peptide

Expression

Bacterial

Mutation

codon optimized for E. coli, residues 22-266

Promoter

T7

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-ERK2-L4A-MEK1_fusion

Plasmid

#39197

Depositor

Melanie Cobb

Publication

Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

Insert

ERK2-MEK1 Fusion (MAP2K1, Mapk1 Rat, Human)

Tags

Myc

Expression

Mammalian

Mutation

L4A = L33A, L37A, L40A, L42A

Promoter

CMV

Available since

July 25, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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