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pCMV-dLight1.1

Plasmid

#111053

Purpose

Expresses dLight1.1 in mammalian cells

Depositor

Lin Tian

Publication

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dLight1.1

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLPC-NMYC TRF2

Plasmid

#16066

Depositor

Titia de Lange

Publication

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Mutation

aa2-500, inserted alanin at aa434

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pmiRFP720-N1

Plasmid

#136560

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720

Depositor

Vladislav Verkhusha

Publication

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

miRFP720

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pSB1C3-J23110-B0034-mRFP1_Magenta

Plasmid

#160446

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Magenta chromoprotein in E. coli

Depositor

Anthony Forster

Publication

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Magenta

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-MAGE-2-429

Plasmid

#89090

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Publication

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only
pQE-30-HyPer3

Plasmid

#42132

Purpose

Genetically encoded H2O2 probe for ratiometric and fluorescence lifetime imaging

Depositor

Vsevolod Belousov

Publication

Bilan et al ACS Chem Biol. 2012 Dec 20.

Insert

HYPER 3

Tags

6His

Expression

Bacterial

Promoter

T5

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pm-iATPSnFR1.0

Plasmid

#102548

Purpose

membrane displayed ATP sensor for mammalian cells

Depositor

Baljit Khakh

Publication

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iATPSnFR1.0

Expression

Mammalian

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHAGE-EFS-MCP-HALOnls

Plasmid

#121937

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Publication

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

MCP-HALOnls

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

EFS

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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CMV-GEX-GECO1

Plasmid

#32443

Purpose

Mammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Publication

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GEX-GECO1.0

Expression

Mammalian

Mutation

GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pN1/G3BP1-iRFP

Plasmid

#129339

Purpose

Stress granule marker

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iRFP (from Dr. M. Matsuda)

Expression

Mammalian

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEGFP-N2-COL1A1

Plasmid

#66602

Purpose

Expresses procollagen 1 alpha 1 as a GFP fusion

Depositor

David Stephens

Publication

Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

COL1A1 (COL1A1 Human)

Expression

Mammalian

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Ca-FLARE (TF)

Plasmid

#92213

Purpose

Transcription factor component of FLARE

Depositor

Alice Ting

Publication

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

NRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16

Use

AAV

Expression

Mammalian

Promoter

synapsin

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PX458-mcherry

Plasmid

#161974

Purpose

Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

Depositor

Joanna Wysocka

Publication

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9-t2a-mcherry

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Publication

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pmScarlet_H2A_C1

Plasmid

#85051

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2A (H2AC16 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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SpyLigase

Plasmid

#51722

Purpose

Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

Depositor

Mark Howarth

Publication

Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

Insert

SpyLigase

Tags

His6 Tag

Expression

Bacterial

Promoter

T7

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBABE HA Chk2 WT

Plasmid

#41901

Depositor

Stephen Elledge

Publication

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

CHK2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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DUSP6

Plasmid

#27975

Depositor

Igor Astsaturov

Publication

Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

Insert

DUSP6 (DUSP6 Human)

Use

Lentiviral

Tags

HA and Myc

Expression

Mammalian

Promoter

CMV

Available since

April 8, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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ASAP2f

Plasmid

#83960

Purpose

Fluorescent reporter for voltage imaging

Depositor

Michael Lin

Publication

Yang et al Cell. 2016 Jun 30;166(1):245-57. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ASAP2f

Expression

Mammalian

Promoter

CAG

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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mEGFP-HRas S17N

Plasmid

#18665

Depositor

Karel Svoboda

Publication

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H-Ras (HRAS Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

S17N mutation in H-Ras

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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