We narrowed to 1,037 results for: pgk promoter

1. pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

   Plasmid

   #187960

   Purpose

   AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

   Plasmid

   #187963

   Purpose

   mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Inserts

   AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
   osTIR1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti HRE mutant-Luc pGK Hygro

   Plasmid

   #118707

   Purpose

   reporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferase

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   HREmutant-Luc
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti PGK Puro GFP-LC3B G120A

   Plasmid

   #123242

   Purpose

   Expression vector with PGK promoter for low expression of EGFP-LC3B G120A. For lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Glycine 120 to Alanine

   Promoter

   PGK

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti PGK Puro GFP-GABARAPL1 G116A

   Plasmid

   #123245

   Purpose

   Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Glycine 116 to Alanine

   Promoter

   PGK

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro

   Plasmid

   #177203

   Purpose

   encoding stblHIF under inducible promoter

   Depositor

   Markus Covert

   Article

   Kudo et al bioRxiv (2022)

   Insert

   stblHIF
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   P402A/P577G/N813A

   Promoter

   TRE

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. EW415 PGK-ZNF35(5-8)-IKZF1(2-3)-realDeimmunLink-NZF (FLP-IN)

   Plasmid

   #236136

   Purpose

   Plasmid encoding the ZNF35/ZNFIKZF1 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoter

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   ZNF35/IKZF1(2-3)-deimmunLink-NZF
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. PLEX-PGK-LAMP1-HaloTag

   Plasmid

   #164216

   Purpose

   pLEX lentivirus backbone expresses HaloTag tagged LAMP1 under PGK promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   PGK

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PLEX-PGK-ANXA11-mCerulean

   Plasmid

   #164214

   Purpose

   pLEX lentivirus backbone expresses mCerulean tagged ANXA11 under PGK promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Lentiviral

   Tags

   mCerulean

   Expression

   Mutation

   Promoter

   PGK

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLEX-PGK-LAMP1-EYFP

    Plasmid

    #164215

    Purpose

    pLEX lentivirus backbone expresses EYFP tagged LAMP1 under PGK promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    PGK

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-EEA-5g-OSK2M2L1-PGK-Puro

    Plasmid

    #102909

    Purpose

    PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2-U6gRNA5(gKAT7-A10)-PGKpuro2ABFP-W

    Plasmid

    #159288

    Purpose

    Lentiviral vector expressing gRNA targeting KAT7 (gRNA ID. A10)

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    guide RNA targeting KAT7 (ID. A10) (KAT7 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro

    Plasmid

    #201677

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassette

    Depositor

    Timo Otonkoski

    Article

    Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

    Insert

    MIR302-3g-EEA-2g-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti PGK Puro GFP-LC3B G120

    Plasmid

    #123241

    Purpose

    Expression vector with PGK promoter for low expression of EGFP-LC3B G120. For lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 121-125. Stop codon after G12…

    Promoter

    PGK

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti PGK FKBP12(F36V)-OGT (Neo)

    Plasmid

    #154294

    Purpose

    Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x HA and FKBP12(F36V)

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti PGK Puro GFP-GABARAPL1 G116

    Plasmid

    #123243

    Purpose

    Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deleted amino acid 117. Stop codon after G116

    Promoter

    PGK

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EW209 PGK-ZNF35(5-8)-ZNF35(5-8)-deImmunLink-NZF(FLP-IN)

    Plasmid

    #236135

    Purpose

    Plasmid encoding the ZNF35/ZNF35 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoter

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    ZNF35(5-8)-ZNF35(5-8)-deImmunLink-NZF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EW196 PGK-ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF(FLP-IN)

    Plasmid

    #236132

    Purpose

    Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoter

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. YH039 PGK-TO-FKBP-tm-GS-XTEN-ADAR2(DD-E488Q-T501A)

    Plasmid

    #233378

    Purpose

    PGK promoter driven FKBP fused with ADAR2dd (E488Q,T501A), with a CD28 transmembrane domain in between

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    FKBP-ADAR2dd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    PGK

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PGKp-GFP-YAP (S94A)

    Plasmid

    #174173

    Purpose

    Lentiviral vector to constitutively express TEAD-binding mutant YAP under control of the human PGK promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    3x FLAG tag

    Expression

    Mammalian

    Mutation

    Serine 94 mutated to Alanine

    Promoter

    PGK

    Available since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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