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shRNA-Ratio-NegCon

Plasmid

#183554

Purpose

Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio negative control (Luciferase), Fig S2)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Luciferase shRNA

Expression

Mammalian

Promoter

CBh

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pFOX11-Tier1-PhCMV-Clover

Plasmid

#169508

Purpose

Tier-1 vector encoding PhCMV-driven gren fluorescent protein clover (PhCMV-clover-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven green fluorescent protein clover

Expression

Mammalian

Promoter

PhCMV

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-TetOn.13

Plasmid

#183584

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 13

Expression

Mammalian

Promoter

Tet-On

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-EF1a.10

Plasmid

#183573

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 10

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-EF1a.11

Plasmid

#183574

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 11

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-EF1a.12

Plasmid

#183575

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 12

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDR-EF1a.5

Plasmid

#183572

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 5

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-EF1a.3

Plasmid

#183570

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 3

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDR-EF1a.1

Plasmid

#183568

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 1

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-CBh.21

Plasmid

#183565

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 21

Expression

Mammalian

Promoter

CBh

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFOX14-Tier1-PhCMV-mTFP1

Plasmid

#169511

Purpose

Tier-1 vector encoding PhCMV-driven green fluorescent protein mTFP1 (PhCMV-mTFP1-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven monomeric cyan fluorescent protein mTFP1

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pFOX15-Tier1-PhCMV-mTurquoise2

Plasmid

#169512

Purpose

Tier-1 vector encoding PhCMV-driven green fluorescent protein mTurquoise2 (PhCMV-mTurquoise2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven monomeric cyan fluorescent protein mTurquoise2

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pFOX12-Tier1-PhCMV-eGFP

Plasmid

#169509

Purpose

Tier-1 vector encoding PhCMV-driven green fluorescent protein eGFP (PhCMV-eGFP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven enhanced green fluorescent protein

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-AKAR (mScarlet-I)

Plasmid

#181861

Purpose

FluoSTEP-AKAR using mScarlet-I as the FRET acceptor.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-AKAR (mScarlet-I)

Tags

mScarlet-I and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0130

Plasmid

#172809

Purpose

Level 1 module containing 35S-CTP-mCHERRY-cYFP_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mCHERRY-cYFP3XHA-3U+TER35S

Use

Synthetic Biology

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0170

Plasmid

#172813

Purpose

Level 1 module containing nCFP_P4 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-SP_SV40-CFP-3U+TERNOS

Use

Synthetic Biology

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0150

Plasmid

#172811

Purpose

Level 1 module containing 35S-CTP-mCHERRY-cVENUS_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mCHERRY-3XFLAGcVENUS-3U+TER35S

Use

Synthetic Biology

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0140

Plasmid

#172810

Purpose

Level 1 module containing 35S-CTP-mCHERRY-nVENUS_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mCHERRY-nVENUS-3U+TER35S

Use

Synthetic Biology

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0120

Plasmid

#172808

Purpose

Level 1 module containing 35S-CTP-mCHERRY-nYFP_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mCHERRY-3XFLAGnYFP-3U+TER35S

Use

Synthetic Biology

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0050

Plasmid

#172801

Purpose

Level 1 module containing 35S-HSP21-cYFP_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-cYFP3XHA-3U+TER35S

Use

Synthetic Biology

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0080

Plasmid

#172786

Purpose

Level 0 module containing NT2_nVenus

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

3X FLAG CtmVENUS 210-238 RSIAT pICH41258 (SP NT2)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0131

Plasmid

#172793

Purpose

Level 0 module containing CDS2ns_deltamPTAC5

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS mature PTAC5 with C-ter delection (41-252), Arabidopsis thaliana

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0010

Plasmid

#172797

Purpose

Level 1 module containing 35S-HSP21-GFP_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-NT2_CTPSSU-mHSP21-GFP-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0020

Plasmid

#172798

Purpose

Level 1 module containing 35S-HSP21-nYFP_notag_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-nYFP-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0030

Plasmid

#172799

Purpose

Level 1 module containing 35S-HSP21-cYFP_notag_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-cYFP-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0040

Plasmid

#172800

Purpose

Level 1 module containing 35S-HSP21-nYFP_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-3XFLAGnYFP-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0060

Plasmid

#172802

Purpose

Level 1 module containing 35S-nYFP-HSP21_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-3XFLAGnYFP-mHSP21-CT_STOP-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0070

Plasmid

#172803

Purpose

Level 1 module containing 35S-cYFP-HSP21_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-cYFP3XHA-mHSP21-CT_STOP-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0080

Plasmid

#172804

Purpose

Level 1 module containing 35S-HSP21-nVENUS_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-nVENUS-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0090

Plasmid

#172805

Purpose

Level 1 module containing 35S-HSP21-cVENUS_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-3XFLAGcVENUS-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0100

Plasmid

#172806

Purpose

Level 1 module containing 35S-PTAC5-cYFP_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mPTAC5-cYFP3XHA-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0110

Plasmid

#172807

Purpose

Level 1 module containing 35S-deltaPTAC5-cYFP_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-deltamPTAC5-cYFP3XHA-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0020

Plasmid

#172776

Purpose

Level 0 module containing NT1_OEP7

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS of chloroplast outer envelope membrane protein OEP7

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1α -NWM-Node 1

Plasmid

#154253

Purpose

expresses reconstructed New World monkey retroCHMP3 Node 1 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed Node 1 of New World monkey retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1α -NWM-Node 2

Plasmid

#154254

Purpose

expresses reconstructed New World monkey retroCHMP3 Node 2 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed Node 2 of New World monkey retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1α -NWM-Node 3

Plasmid

#154255

Purpose

expresses reconstructed New World monkey retroCHMP3 Node 3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed Node 3 of New World monkey retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Mito-CoilE-mEm

Plasmid

#181994

Purpose

CoilE genetically encoded tag on Mito-mEmerald, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

Mito-CoilE-mEm

Tags

mEmerald

Expression

Mammalian

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TOMM20-CoilE-mCherry

Plasmid

#181995

Purpose

CoilE genetically encoded tag on TOMM2--mCherry, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

TOMM20-CoilE-mCherry

Tags

mCherry

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB-NEO-PYL

Plasmid

#114393

Purpose

For PYL expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL

Tags

3xFLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBN086

Plasmid

#173448

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y715E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y715E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y715E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 9561 - 9600 of 23042 results

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