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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 27,822 results for: LEA

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Showing: 4781 - 4800 of 27822 results

  2. pαH-S-GSAS-2P-OMICRON

    Plasmid
    #180593
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180593-plasmid-map-sequence-id-352871

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  12. pTC223

    Plasmid
    #70019
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Expression
    Plant
    Available since
    Dec. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70019-plasmid-map-sequence-id-124558

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  16. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

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  17. LVXN-Neo-NSD2-E1099K

    Plasmid
    #86011
    Purpose
    express NSD2 E1099K mutant in mammalian cells
    Depositor
    Darrin Stuart
    Publication
    Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NSD2 E1099K mutant (NSD2 Human)
    Tags
    Flag tag  D Y K D D D D K
    Expression
    Mammalian
    Mutation
    E1099K; K1002R (please see depositor comments bel…
    Promoter
    CMV
    Available since
    Jan. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 4781 - 4800 of 27822 results