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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,403 results for: LEA

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Showing: 4781 - 4800 of 23403 results

  4. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

    Plasmid
    #181744
    Purpose
    bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY-HF1
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…
    Promoter
    T7
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    181744-plasmid-map-sequence-id-373171

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  10. pCDNA5_FRT_TO_TurboID-mCherry-Cry2

    Plasmid
    #166504
    Purpose
    Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.
    Depositor
    Angelos Constantinou
    Article
    Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2
    Insert
    None
    Tags
    TurboID and mCherry-CRY2
    Expression
    Mammalian
    Available Since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166504-plasmid-map-sequence-id-328214

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  16. pLenti4/TO-hEPO

    Plasmid
    #50437
    Purpose
    express human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery system
    Depositor
    Juan Melero-Martin
    Article
    Lin et al Blood. 2011 Nov 17;118(20):5420-8. doi:
    Insert
    EPO (EPO Human)
    Use
    Lentiviral; Tetracycline-regulatable (t-rex)
    Expression
    Mammalian
    Promoter
    CMV/TO
    Available Since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. SpCas9-HF1

    Plasmid
    #138556
    Purpose
    Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-HF1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138556-plasmid-map-sequence-id-274763

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  20. pYPQ166

    Plasmid
    #109328
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter
    Depositor
    Yiping Qi
    Article
    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.
    Insert
    zCas9
    Use
    CRISPR; Gateway compatible zcas9 entry clone
    Tags
    3x FLAG, NLS and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Available Since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    109328-plasmid-map-sequence-id-210215

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Showing: 4781 - 4800 of 23403 results