We narrowed to 119,800 results for: %s

1. px335 Mettl14 sgRNA #2

   Plasmid

   #61514

   Purpose

   encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

   Depositor

   Jacob Hanna

   Article

   Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

   Insert

   gccgctcccggatctcctgc
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pENTR1A-Yap S127A

   Plasmid

   #46050

   Depositor

   Xin Chen

   Article

   Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Entry vector

   Tags

   2X Flag tag

   Mutation

   S127A

   Available Since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

3. px335 Mettl14 sgRNA #1

   Plasmid

   #61515

   Purpose

   encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

   Depositor

   Jacob Hanna

   Article

   Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

   Insert

   gcggcagctcctagctcagc
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pDONR221-ACE2 receptor 19-615

   Plasmid

   #167012

   Purpose

   Gateway-compatible Entry vector encoding ACE2 receptor (aa19-615) interacting with spike (S1) RBD domain from SARS-CoV-2

   Depositor

   Spyros Petrakis

   Article

   Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

   Insert

   ACE2 (ACE2 Human)
   

   Expression

   Mammalian

   Mutation

   Amino acids 19-615, No stop codon

   Available Since

   March 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pML390_YWHAQ-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227925

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   YWHAQ homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pML389_YWHAB-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227926

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   YWHAB homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pML600_COG8-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227911

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   COG8 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pML357_CAV1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227914

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   CAV1 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pML361_DCP1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227918

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   DCP1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pML366_HSPA1B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227920

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    HSPA1B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pML365_HSP90AA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227921

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    HSP90AA homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pML364_PSMB7-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227922

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    PSMB7 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pML374_CLTA-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227893

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    CLTA homology arms with mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pML307_SEC61B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227894

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    SEC61B homology arms with mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pML383_CCDC47-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227896

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    CCDC47 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pML384_NCLN-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227897

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    NCLN homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pML318_SEC23A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227903

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    SEC23A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL4.23-hs737

    Plasmid

    #222472

    Purpose

    Luciferase vector containing the hs737 enhancer sequence (reference sequence).

    Depositor

    Tychele Turner

    Article

    Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:

    Insert

    hs737 enhancer (reference sequence) (LOC110120928 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_SEPT9_i3 Gmut-msfGFP

    Plasmid

    #180326

    Purpose

    mammalian expression of human SEPT9_i3 Gmut fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN9_v3 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i3 W502A, H512D

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP

    Plasmid

    #180311

    Purpose

    bacterial expression of human SEPT2 NCmut fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    SEPT2 F20D, V27D

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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