We narrowed to 25,085 results for: Mut

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pCS2-Cas9

Plasmid

#47322

Purpose

For in vivo expression of Cas9 or generation of Cas9 mRNA

Depositor

Alex Schier

Article

Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

Insert

Cas9

Use

CRISPR; Xenopus and zebrafish expression

Expression

Mammalian

Promoter

SP6

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-beta-catenin-S33Y

Plasmid

#13371

Purpose

Retroviral expression of mouse beta-catenin S33Y mutant

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

catenin (cadherin associated protein), beta 1 (S33Y mutant) (Ctnnb1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Serine 33 to Tyrosine and Leucine 388 to Proline

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pRetroX GFP T2A Cre

Plasmid

#63704

Purpose

Inducible expression of GFP T2A Cre fusion in mammalian cells

Depositor

Floris Foijer

Article

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

T2A Cre

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL11059

Plasmid

#68263

Purpose

Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro1 T18N

Plasmid

#47890

Purpose

Expresses myc tagged Miro1 T18N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 T18N (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

T18N, dominant negative mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ARAF

Plasmid

#51076

Purpose

mammalian expression of wt ARAF

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 deltaN47 Beta-catenin

Plasmid

#19287

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

N-terminal truncation, deleted aa 1-47

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21-Q76L (CA)

Plasmid

#83422

Purpose

Mouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Residue 76 from Q to L (Q76L: CAG -> CTG)

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAdSh.PGK.Cas9

Plasmid

#58253

Purpose

Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

Human codon-optimized S. pyogenes Cas9

Use

Adenoviral and CRISPR

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pMA3287

Plasmid

#46883

Purpose

Lentiviral vector encoding Tet-inducible mitochondrially targeted Y147A mutant human Uracil-N-glycosylase

Depositor

Mikhail Alexeyev

Article

Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

Insert

Uracil-N-glycosylase, Y147A mutant (UNG Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Y147A

Promoter

TRE-Tight

Available Since

Aug. 14, 2013

Availability

Academic Institutions and Nonprofits only

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sq5

Plasmid

#24499

Depositor

Bruce Conklin

Article

Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

Insert

Gs alpha (Gnas Rat)

Tags

HA (see comments)

Expression

Mammalian

Mutation

This is Gs alpha with the C-terminal amino acids …

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-Rab21-T31N (DN)

Plasmid

#83423

Purpose

Mouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Residue 31 from T to N (T31N: ACG -> AAC)

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-NT-3/D15A

Plasmid

#73035

Purpose

Express an NT-3-BDNF hybrid neurotrophic factor in mammalian cells

Depositor

Pantelis Tsoulfas

Article

Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

Insert

NT-3/D15A (NTF3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Mutated Aspartic acid in position 15 of the matur…

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro1 P13V

Plasmid

#47889

Purpose

Expresses myc tagged Miro1 P13V constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 P13V (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

P13V, constitutively active mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pCI-Neo-PTPRT (WT)

Plasmid

#16630

Depositor

Bert Vogelstein

Article

Wang et al Science. 2004 May 21. 304(5674):1164-6.

Insert

PTPRT (PTPRT Human)

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pSKA413

Plasmid

#80381

Purpose

Constitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.

Depositor

Mustafa Khammash

Article

Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

Inserts

ccaS (complement)
ccaR (complement)
gfpmut3

Use

Synthetic Biology; Optogenetics

Tags

FLAG tag

Expression

Bacterial

Promoter

ccaR and cpcG2Δ59

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV5-rat ERK2-L73P/S151D

Plasmid

#40819

Depositor

Natalie Ahn

Article

Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

Insert

MAPK1 (Mapk1 Rat)

Tags

His6

Expression

Mammalian

Mutation

L73P, S151D

Promoter

CMV

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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6691 Bicistronic_ires_puro

Plasmid

#64335

Purpose

This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Type

Empty backbone

Use

Retroviral

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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CAV1-K*R-mEGFP

Plasmid

#27766

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1, lysine null mutant (CAV1 Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

All lysines mutated to arginines (K*R)

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(D323A/D325A)

Plasmid

#86592

Purpose

Expresses human full-length Akt1(D323A/D325A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

D323A/D325A transforming mutations

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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