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pET28a(CTLA4)

Plasmid

#177905

Purpose

Bacterial expression of his10-tagged human CTLA4 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

CTLA4 (aa 183-223 only) (CTLA4 Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR

Plasmid

#135500

Purpose

Mammalian expression of FLAG-tagged TAPBPR

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

TAPBPR (TAPBPL Human)

Tags

Luminal FLAG epitope tag and Signal peptide from …

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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gh38

Plasmid

#106712

Purpose

expression of gRNA targeting STT3A

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

STT3A (STT3A Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RIαΔ(D/D+linker)-EGFP

Plasmid

#181843

Purpose

GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-(D/D+linker)EGFP (PRKAR1A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of residues 12-112 in PKA RIα.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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EGFPC2-CB2 SH3-

Plasmid

#68822

Purpose

EGFP tagged Collybistin expression in mammalian cells

Depositor

Shiva Tyagarajan

Article

de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

Insert

Collybistin (Arhgef9 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Zn2

Plasmid

#174805

Purpose

Bacterial expression of isolated PARP1 Zn2 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Zn2 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Zn3

Plasmid

#174806

Purpose

Bacterial expression of isolated PARP1 Zn3 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Zn3 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-dddAI

Plasmid

#186559

Purpose

Broad host range regulated expression of the DddA immunity determinant (DddAI)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Expression

Bacterial

Promoter

tac

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

Plasmid

#192789

Purpose

aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

antiGFP nanobody minimizer

Tags

H6 and ybbR

Expression

Bacterial

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-FLJ10081

Plasmid

#64775

Purpose

Mammalian expression of Flag-tagged FLJ10081 (NSL3)

Depositor

Joan Conaway, Ronald Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

FLJ10081 (KANSL3 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delBRCT

Plasmid

#173938

Purpose

Bacterial expression of PARP1 lacking BRCT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delBRCT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 374-520; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 2xSTREP Cyclin F(LP35/36AA)

Plasmid

#236467

Purpose

transient overexpression of Cyclin F in mammalian cells

Depositor

Michele Pagano

Article

D'Angiolella et al Cell. 2012 May 25;149(5):1023-34. doi: 1

Insert

Cyclin F (CCNF Human)

Tags

2xSTREP

Expression

Mammalian

Mutation

(LP35/36AA) first two amino acids of the F-box do…

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-KIAA0947

Plasmid

#49248

Purpose

Mammalian expression of flag-tagged human KIAA0947

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

KIAA0947 (ICE1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5-Flag-Med26

Plasmid

#49250

Purpose

Mammalian expression of flag-tagged Med26

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med 26 (MED26 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC

Plasmid

#114377

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targeting

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )

Use

AAV

Tags

EYFP and Kv2.1C-linker-TlcnC

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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LRRC3_pcDNA6.2/EmGFP-Bsd

Plasmid

#176972

Purpose

Mammalian expression vector encoding LRRC3 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

LRRC3 (LRRC3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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hSyn-DIO-BiPOLES-mCerulean

Plasmid

#154950

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Expression

Mammalian

Promoter

human synapsin

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-SARS-CoV-2-3CL-C145A

Plasmid

#160279

Purpose

Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C145A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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AT-U1

Plasmid

#112059

Purpose

U1 snRNA tagged with 1 copy of Riboglow (truncated variant A)

Depositor

Amy Palmer

Article

Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

Insert

U1 (RNU1-1 Human)

Expression

Mammalian

Mutation

1 copy of Riboglow tag (truncated variant A) near…

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pPSV39-ugi

Plasmid

#186556

Purpose

Broad host range inducible expression of uracil-DNA glycosylase inhibitor (UGI)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

ugi

Expression

Bacterial

Promoter

LacUV5

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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