We narrowed to 19,249 results for: tec

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pT7-ZoAl-ENT-3xF-ORF

Plasmid

#238484

Purpose

For production of C-term flag-tagged ZoAl EN-tuned ORF mRNA for mRNA transfection in PRINT

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement mRNA

Use

Ivt template

Tags

3x-Flag

Mutation

R1103A

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Lifeact-(GGS)X6-HaloTag C58

Plasmid

#171435

Purpose

Expression of Lifeact-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

Lifeact-(GGS)X6-HaloTag C58

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pA5c-tesA-vhb50-8fadR

Plasmid

#86601

Purpose

expression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoter

Depositor

Yinjie Tang

Article

Liu et al Biotechnol Bioeng. 2016 Aug 6. doi: 10.1

Inserts

tesA
Vhb50
fadR

Expression

Bacterial

Promoter

LacUV5 and pBAD

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.2

Plasmid

#208307

Purpose

Expresses ABE-RA4.2 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.2-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ND4.2-Left TALE-G1397-N-wildtype

Plasmid

#179684

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N_1xUGI_P2A_mCherry_ATP5B 3'UTR

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAC1430

Plasmid

#127981

Purpose

AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

Cpf1

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

codon optimized for Aspergillus nidulans

Promoter

Aspergillus nidulans tef1 promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMT451

Plasmid

#139391

Purpose

Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4), pNBU1 backbone, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4)

Use

Synthetic Biology

Promoter

BA inducible promoters

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pBT100.106b

Plasmid

#122601

Purpose

clone APOBEC1 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

APOBEC1

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19/mChe-Px

Plasmid

#183170

Purpose

Construct for transient expression of peroxisomes-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

mCherry-Px

Tags

10 aa peroxisome targeting signal 1 (IHHPRELSRL) …

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJL-SaBE3

Plasmid

#85169

Purpose

Expresses SaBE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

SaBE3

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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UPB-pancreas (UPB7)

Plasmid

#180787

Purpose

Unique Projection Barcode (UPB7)-Pancreas for Projection-seq

Depositor

Rui Chang

Article

Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

Insert

hM4D(Gi) (partial )

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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EK0304 CMV-TO-BFPmut-stop-Bdnf.t1 (FLP-IN)

Plasmid

#191146

Purpose

Expression of only the trigger sequence for Bdnf.s1 (EK0301) in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

Bdnf.t1

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSCARS1

Plasmid

#174868

Purpose

Contains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1

Depositor

Zengyi Shao

Article

Lopez et al Appl Microbiol Biotechnol. 2021 Aug 6. p

Insert

ARS1

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19/Pd-mChe

Plasmid

#183172

Purpose

Construct for transient expression of plasmodesmata-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

Pd-mCherry

Tags

AtPDCB1, A. thaliana PLASMODESMATA CALLOSE-BINDIN…

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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CJ8421_04975 Rptr2 and CJ8421_04975 mRNA

Plasmid

#194210

Purpose

Expresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 mRNA and CJ8421_04975 Rptr2

Expression

Bacterial

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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ND4.2-Right DdCBE-G1397-C-wildtype

Plasmid

#179687

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C )_1xUGI_SOD2 3'UTR

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA

Plasmid

#194247

Purpose

Expresses 3 miRNAs targeting human alpha-Synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

miRaSynuclein(human) (SNCA Human)

Use

AAV and RNAi

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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LbCas12a-D156R

Plasmid

#213744

Purpose

For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

LbCas12a

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJIT163-2NLSCas9

Plasmid

#53064

Purpose

Expression of rice codon-optimized Cas9 in plant cells

Depositor

Caixia Gao

Article

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

Cas9

Use

Plant expression

Mutation

Rice codon optimized

Promoter

2x35S

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pVH41-Tier1-PhCMV-GLuc

Plasmid

#169544

Purpose

Tier-1 vector encoding PhCMV-driven gaussia luciferase (PhCMV-GLuc-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven Gaussia Luciferase

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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