We narrowed to 14,429 results for: nts

1. pMOD_A0908

   Plasmid

   #91027

   Purpose

   Module A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TREX2-P2A-AtCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EC1.2

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCK671.306

   Plasmid

   #192641

   Purpose

   Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Insert

   J306 scRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   BBa_J23119

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-HA-ERK2 L232A

   Plasmid

   #8976

   Depositor

   John Blenis

   Article

   Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

   Insert

   ERK2 (Mapk1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   L232A

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

4. MKK7

   Plasmid

   #29576

   Depositor

   Dustin Maly

   Article

   Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

   Insert

   MKK7 (Map2k7 Mouse)
   

   Use

   Tags

   GST, HIS6, and TEV

   Expression

   Bacterial

   Mutation

   Starting Met removed

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pDGE8

   Plasmid

   #79463

   Purpose

   sgRNA shuttle vector; module 2E

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   pAtU6-ccdB-sgRNA (ccdB(letD) )
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-DUET-1-mRhubarb719-HO

   Plasmid

   #141200

   Purpose

   Expresses mRhubarb719 fluorescent protein and heme oxygenase

   Depositor

   Elad Firnberg

   Article

   Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

   Inserts

   mRhubarb719
   heme oxygenase
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

   Promoter

   T7

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-HA-ERK2 Y261A

   Plasmid

   #8977

   Depositor

   John Blenis

   Article

   Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

   Insert

   ERK2 (Mapk1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y261A

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pFH49

   Plasmid

   #128211

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCTCON2-GYB

   Plasmid

   #160927

   Purpose

   Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Inserts

   BFP
   sfGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

    Plasmid

    #31478

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV HA NLS Y2 K227M T2A BFP
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    F13Y, S111Y, K227M = Y2 nickase "reo" …

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDH3

    Plasmid

    #83789

    Purpose

    Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    CFP
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    F64L, Y66W, N146I, M153T, V163A, N164H mutations …

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAtCas9_1

    Plasmid

    #91156

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. XE68 APC-GFP in CS2+

    Plasmid

    #16687

    Depositor

    Randall Moon

    Article

    Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

    Insert

    APC-GFP (APC Human)
    

    Use

    Xenopus expression

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pRS416-Aga1p-FAPB2.3.6

    Plasmid

    #158147

    Purpose

    Yeast display reporter for non-display yeast strains (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p-FAPB2.3.6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LLP689_L2_1IF2_FRT-OCS_Rluc_C1_FlpF2

    Plasmid

    #192412

    Purpose

    To act as a 1-input state of the split-AND gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLS

    Plasmid

    #192413

    Purpose

    To act as a 1-input state of the split-AND gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMOD_A0503

    Plasmid

    #91013

    Purpose

    Module A, Promoter: CmYLCV, Gene: Csy4-P2A-AtCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CmYLCV

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBTK1023 (pDS-InR1)

    Plasmid

    #183130

    Purpose

    Expresses dsRNA targeting the InR1 gene from Apis mellifera

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al Science. 2020 Jan 31;367(6477):573-576.

    Insert

    Fragment of InR1 gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pTX198

    Plasmid

    #89262

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsPDS-gRNA01
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Maize Ubiquitin1 promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpyTag-GlpG

    Plasmid

    #79054

    Purpose

    Expresses SpyTagged GlpG protein

    Depositor

    James Bowie

    Article

    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

    Insert

    SpyTag-GlpG (glpG )
    

    Use

    Tags

    6xHis and SpyTag

    Expression

    Bacterial

    Mutation

    GlpG protein employed here corresponds to residue…

    Promoter

    Trc

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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