We narrowed to 73,658 results for: LAS

1. pSMART-sgRNA (Sp)

   Plasmid

   #80427

   Purpose

   U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

   Depositor

   Melissa Little, Sara Howden

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-DHFR

   Plasmid

   #80329

   Purpose

   Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Insert

   DHFR-TSc3
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   DHFR-TS 5' UTR

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. M4786 KanMX::hisG-URA3-HisG Disruptor Converter

   Plasmid

   #51690

   Purpose

   Yeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisG

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   hisG-URA3-hisG (URA3 Budding Yeast)
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGCaMP5-DHFR

   Plasmid

   #78706

   Purpose

   GCaMP5 under the Toxoplasma SAG1 5' UTR. Encodes a DHFR selectable marker.

   Depositor

   Sebastian Lourido

   Article

   Sidik et al J Biol Chem. 2016 Apr 29;291(18):9566-80

   Inserts

   GCaMP5
   DHFR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Toxoplasma SAG1 5' UTR

   Available since

   May 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT3-RhoGC

   Plasmid

   #100031

   Purpose

   This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein.

   Depositor

   Daniel Oprian

   Article

   Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

   Insert

   RhoGC
   

   Use

   Tags

   1D4 T9'A (AETSQVAPA) and C8 (PRGPDRPEGIEE)

   Expression

   Mammalian

   Mutation

   Promoter

   Adenovirus major late promoter

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMflT-o2

   Plasmid

   #101310

   Purpose

   Contains: oriC2 fragment of M. florum L1 replication origin (dnaA/dnaN intergenic region), colE1 replication origin, recoded tetM resistance gene.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Insert

   oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTUB1:OsTIR1-3FLAG, SAG1:CAT

   Plasmid

   #87258

   Purpose

   TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.

   Depositor

   David Sibley

   Article

   Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

   Inserts

   TIR1
   CAT
   

   Use

   Toxoplasma expression

   Tags

   3FLAG

   Expression

   Mutation

   Codon optimized for Toxoplasma gondii expression

   Promoter

   TgSAG1 361 bp and TgTUB1 2717 bp

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT

   Plasmid

   #87259

   Purpose

   YFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133

   Depositor

   David Sibley

   Article

   Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

   Inserts

   YFP-mAID-3HA
   HXGPRT
   

   Use

   Toxoplasma expression

   Tags

   3HA and mAID

   Expression

   Mutation

   Codon optimized for Toxoplasma gondii expression

   Promoter

   TgDHFR-TS 463 bp and TgTUB1 378 bp

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUF1-dCas9-GCN5

   Plasmid

   #122509

   Purpose

   Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.

   Depositor

   Lubin Jiang

   Article

   Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

   Insert

   dCas9-GCN5
   

   Use

   CRISPR

   Tags

   3*FLAG

   Expression

   Mutation

   dCas9(D10A,H840A)

   Promoter

   PfHsp86

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUF1-dCas9-Sir2a

    Plasmid

    #122510

    Purpose

    Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.

    Depositor

    Lubin Jiang

    Article

    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

    Insert

    dCas9-Sir2a
    

    Use

    CRISPR

    Tags

    3*FLAG

    Expression

    Mutation

    dCas9(D10A,H840A)

    Promoter

    PfHsp86

    Available since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAT15415-BEAR-GFP-target-mCherry

    Plasmid

    #162995

    Purpose

    plasmid expressing an sgRNA targeting the BEAR-GFP plasmid along with an mCherry marker

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    sgRNA targeting the BEAR-GFP plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGM55

    Plasmid

    #71249

    Purpose

    luciferase reporter with hGM-CSF -55 to +28 minimal promoter

    Depositor

    Peter Cockerill

    Article

    Bert et al Plasmid. 2000 Sep;44(2):173-82.

    Insert

    GM-CSF minimal promoter (-55 to +28) (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GM-CSF minimal

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAT15416-BEAR-mScarlet-target-BFP

    Plasmid

    #162996

    Purpose

    plasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP marker

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    sgRNA targeting the BEAR-mScarlet plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT3-RhoGC E254D

    Plasmid

    #100033

    Purpose

    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.

    Depositor

    Daniel Oprian

    Article

    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

    Insert

    RhoGC E254D
    

    Use

    Tags

    1D4 (TETSQVAPA)

    Expression

    Mammalian

    Mutation

    E254D

    Promoter

    Adenovirus major late promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pMT3-RhoGC D380E

    Plasmid

    #100035

    Purpose

    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 533 nm.

    Depositor

    Daniel Oprian

    Article

    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

    Insert

    RhoGC D380E
    

    Use

    Tags

    1D4 (TETSQVAPA)

    Expression

    Mammalian

    Mutation

    D380E

    Promoter

    Adenovirus major late promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT3-RhoGC D380N

    Plasmid

    #100034

    Purpose

    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 506 nm.

    Depositor

    Daniel Oprian

    Article

    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

    Insert

    RhoGC D380N
    

    Use

    Tags

    1D4 (TETSQVAPA)

    Expression

    Mammalian

    Mutation

    D380N

    Promoter

    Adenovirus major late promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCTIP2-N106

    Plasmid

    #66808

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses mouse CTIP2 with blasticidin resistance

    Depositor

    Andrew Yoo

    Article

    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

    Insert

    CTIP2 isoform b (Bcl11b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-MHL: nsp16_SARS-CoV-2

    Plasmid

    #167850

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp16

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp16
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pET28-MHL: nsp10_SARS-CoV-2

    Plasmid

    #167851

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp10

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. FH1-MGT#1-mVenus

    Plasmid

    #164094

    Purpose

    Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

    Depositor

    Gaetano Gargiulo

    Article

    Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

    Insert

    MGT#1-mVenus
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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