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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,173 results for: CAG

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Showing: 4941 - 4960 of 10173 results

  2. Level 1 P4 TaU6 guide acceptor

    Plasmid
    #165600
    Purpose
    GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid
    Depositor
    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
    Publication
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TaU6 promoter::LacZ::sgRNA scaffold
    Use
    CRISPR and Synthetic Biology; Sgrna acceptor with…
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165600-plasmid-map-sequence-id-331273

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  3. Level 1 P3 TaU6 guide acceptor

    Plasmid
    #165599
    Purpose
    GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid
    Depositor
    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
    Publication
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TaU6 promoter::LacZ::sgRNA scaffold
    Use
    CRISPR and Synthetic Biology; Sgrna acceptor with…
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165599-plasmid-map-sequence-id-331270

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  4. pEdit

    Plasmid
    #232355
    Purpose
    The pEdit series plasmids are derived from the pTarget series plasmids by inserting homologous recombination arms targeting the gene of interest, which enables gene knockout or gene insertion at the t
    Depositor
    Yujie Cai
    Publication
    Gou et al Biotechnol Bioeng. 2025 May;122(5):1233-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA
    Use
    CRISPR
    Available since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    232355-plasmid-map-sequence-id-485174

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  5. ATP1A1_G4_Q118R_v2_Dual_epegRNA_tevopreQ1

    Plasmid
    #187456
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4_v2 optimized epegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G4 Q118R_v2 epegRNA (tevopreQ1) (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187456-plasmid-map-sequence-id-367464

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  6. pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry

    Plasmid
    #177182
    Purpose
    plasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry marker
    Depositor
    Ervin Welker
    Publication
    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pegRNA targeting the PEAR-GFP plasmid
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    177182-plasmid-map-sequence-id-348475

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  7. pmIL-6 mut C/EBP

    Plasmid
    #61291
    Purpose
    drives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) site
    Depositor
    Gail Bishop
    Publication
    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IL-6 promoter (Il6 Mouse)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Mutated C/EBP binding site from ACATTGTGCAATCT to…
    Promoter
    IL-6 promoter with mutant C/EBP binding site
    Available since
    May 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  10. px335 Mettl14 sgRNA #1

    Plasmid
    #61515
    Purpose
    encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)
    Depositor
    Jacob Hanna
    Publication
    Geula et al Science. 2015 Feb 27;347(6225):1002-6. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gcggcagctcctagctcagc
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Jan. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid
    #181745
    Purpose
    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1
    Depositor
    Benjamin Kleinstiver
    Publication
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    human/zebrafish codon optimized SpCas9
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    181745-plasmid-map-sequence-id-354676

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  14. ATP1A1_G8_Dual_sgRNA

    Plasmid
    #178104
    Purpose
    Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G8 sgRNA + user-specified sgRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178104-plasmid-map-sequence-id-350193

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  20. pLXIN2-RFP

    Plasmid
    #99205
    Purpose
    RFP expressing bicistronic retroviral vector
    Depositor
    Saroj Mathupala
    Insert
    Red fluorescent protein
    Use
    Retroviral; Bicistronic retroviral expression vec…
    Expression
    Mammalian
    Mutation
    The dsRed cDNA was PCR amplified off AddGene plas…
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    99205-plasmid-map-sequence-id-192441

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Showing: 4941 - 4960 of 10173 results