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EYFP-Clathrin

Plasmid

#20921

Depositor

Xiaowei Zhuang

Article

Rust et al Nat Struct Mol Biol. 2004 Jun . 11(6):56

Insert

Clathrin, light polypeptide (Lca) (Clta Mouse)

Tags

EYFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
RhoC FLARE.sc mCer, mVenus - CA

Plasmid

#65424

Purpose

RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, Q63L in RhoC

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
RhoC FLARE.sc mCer, mVenus - DN

Plasmid

#65423

Purpose

RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, T19N in RhoC

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
pFBD-ESCRT 0

Plasmid

#21499

Depositor

James Hurley

Article

Ren et al Structure. 2009 Mar 11. 17(3):406-16.

Insert

pFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Mouse, Human)

Tags

GST and His-MBP

Expression

Insect

Mutation

none

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

Plasmid

#183069

Purpose

Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c_4

Use

CRISPR

Tags

His10 and MBP

Expression

Bacterial

Mutation

Wild-type

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pFosill-4

Plasmid

#89698

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR H215R

Plasmid

#13509

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR H215R (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Mutation

H215R

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only
pNAS1B split mCherry N-link/link-C

Plasmid

#61965

Purpose

Negative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)

Depositor

Lynne Regan

Article

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

N-mCherry-link
link-C-mCherry

Expression

Bacterial

Promoter

PBAD and PLtetO

Available Since

Feb. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pPLtetO-ClpX

Plasmid

#89657

Purpose

Plasmid for titrating synthesis of ClpX with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

clpX

Use

Synthetic Biology

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT005

Plasmid

#182715

Purpose

Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

CasRx crRNA cloning backbone

Use

CRISPR

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCas_5+7_gfp

Plasmid

#60595

Purpose

encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_5+7_gfp

Expression

Bacterial

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pSG424 Smad2 (Gal4-Smad2)

Plasmid

#14932

Depositor

Joan Massague

Article

Liu et al Genes Dev. 1997 Dec 1. 11(23):3157-67.

Insert

Smad2 (SMAD2 Human)

Tags

Gal4

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pCFJ1272

Plasmid

#44486

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, MCS

Use

Multiple cloning site vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pTAJAK-71 (pESC-NatMXsyn-USER)

Plasmid

#78232

Purpose

Empty vector, for the insertion of gRNA expression cassettes into the vector.

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-miR9/9*

Plasmid

#177805

Purpose

The pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-9/9*

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pReporter_5

Plasmid

#60566

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_5

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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3539 pMIG Bax

Plasmid

#8788

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bax (Bax Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pBlastR-12xLexAOp-mCherry-MiniWhite

Plasmid

#165917

Purpose

Blasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable LexAOp Response Vector

Use

Synthetic Biology

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-R4-uAb

Plasmid

#101801

Purpose

Bacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

Depositor

Matthew DeLisa

Article

Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

Insert

R4-uAb

Tags

6xHis and Flag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pIntegrase_10

Plasmid

#60581

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_10

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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