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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,383 results for: Coli

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Showing: 7441 - 7470 of 10383 results

  11. pGDP4 tet(A)

    Plasmid
    #112887
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    tet(A)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Sequence was codon optimized for E. coli K12 usin…
    Promoter
    Plac
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112887-plasmid-map-sequence-id-219894

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  15. pCP60 RS-GFP

    Plasmid
    #122173
    Purpose
    "Binary vector for Agrobacterium tumefacience-mediated transformation of plant cells resulting in constitutive expression of cytoplasmatically localized RS-GFP"
    Depositor
    Lukas Fischer
    Article
    Nocarova et al BMC Plant Biol. 2009 Apr 22;9:44. doi: 1
    Insert
    RS-GFP
    Use
    Binary vector for escherichia coli and agrobacter…
    Expression
    Plant
    Promoter
    CaMV 35S
    Available Since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    122173-plasmid-map-sequence-id-238572

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  16. pCAT-YFP-TetR

    Plasmid
    #59018
    Purpose
    Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii
    Depositor
    David Sibley
    Article
    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537
    Insert
    YFP-TetR
    Use
    Toxoplasma expression
    Promoter
    alpha-tubulin
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59018-plasmid-map-sequence-id-137741

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  18. pPP381

    Plasmid
    #158798
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-2
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    RuvC-inactivating mutation D394A
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158798-plasmid-map-sequence-id-309523

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  19. pHBintT

    Plasmid
    #48134
    Purpose
    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector
    Depositor
    Dieter Jahn
    Article
    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48134-plasmid-map-sequence-id-69938

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  20. pRAN473

    Plasmid
    #120813
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1
    Insert
    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    Expression
    Bacterial
    Promoter
    Ptet
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120813-plasmid-map-sequence-id-235308

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  21. pMesh1*

    Plasmid
    #175591
    Purpose
    Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Mesh1 (Mesh1 Fly)
    Tags
    Fused with a flexible GS linker to cerulean
    Expression
    Bacterial
    Mutation
    Codon optimized for translation in E.coli. AGGAGG…
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175591-plasmid-map-sequence-id-348365

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  22. pENTR_L1-YFP-miniTurbo-L2

    Plasmid
    #127352
    Purpose
    gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, V5, and YFP
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127352-plasmid-map-sequence-id-246314

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Showing: 7441 - 7470 of 10383 results