We narrowed to 10,112 results for: CAG
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Plasmid#58217PurposeRatiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGEM-CEPIA1er
TagsmycExpressionMammalianMutationGEM-GECO1 E298D F359W D400E E407DPromoterCAGAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJRH-1187 VSVGmut
Plasmid#201913PurposeExpresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)DepositorInsertVSV-G (K47Q, R354A)
ExpressionMammalianMutationK47Q, R354APromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSaCas9_GFP
Plasmid#64709PurposeCo-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSaCas9-2A-GFP
UseCRISPRTags2A-GFP and 3xFLAG-SV40 NLSExpressionMammalianPromoterCAGAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-FLEX-Arch-GFP
Plasmid#22222PurposeCre-dependent viral expression of Arch-GFP for neuronal inhibitionDepositorPublicationHas serviceAAV1 and AAV9InsertArch
UseAAV and Cre/Lox; Adeno-associated virusTagsGFPExpressionMammalianMutationnoPromoterCAGAvailable sinceJan. 6, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJRH-1362 scFv entry plasmid
Plasmid#201912PurposeEntry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)DepositorInsertStuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
ExpressionMammalianPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
tFucci(SA)5
Plasmid#153520PurposeNew color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttFucci(SA)5.
TagsAzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)ExpressionBacterial and MammalianPromoterCAGAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pgRNA-CKB
Plasmid#73501PurposegRNA expression vector (with mKate2)DepositorInsertmKate2-T2A-Bsd
UseCRISPR and LentiviralTagsNLSExpressionMammalianPromoterCAGAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJRH029
Plasmid#171060PurposePlasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in betweenDepositorInsertGag-spyCas9
UseCRISPR and LentiviralTags2x SV40 NLS and 3x FLAG tagExpressionMammalianPromoterCAGAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAVS1-PDi-CRISPRn
Plasmid#73500PurposeDox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
rtTA
UseCRISPRTags3xFLAG and NLSExpressionMammalianPromoterCAG and TREAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEX-PK-hLC3
Plasmid#61458PurposeExpress pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagyDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPK-hLC3 (MAP1LC3B Human, Fission Yeast)
ExpressionMammalianPromoterCAGAvailable sinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-FLEX-rev-ChR2(H134R)-mCherry
Plasmid#18916PurposeCre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitationDepositorInsertChannelrhodopsin 2-mCherry
UseAAV and Cre/Lox; Adeno-associated virusTagsmCherryMutationH134RPromoterCAGAvailable sinceJan. 29, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAFNF-GFP
Plasmid#13772PurposeFlp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.DepositorInsertEGFP
UseFlp/frtExpressionMammalianPromoterCAGAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSQT834
Plasmid#53371PurposeCsy4 and Cas9 nuclease expression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCsy4-T2A-Cas9-NLS
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCBASceI
Plasmid#26477PurposeI-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI siteDepositorInsertpCBASceI
TagsHAExpressionMammalianAvailable sinceOct. 20, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAVS1-NC-CRISPRi (Gen3)
Plasmid#73499PurposeConstitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-KRAB
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAGAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SQT1659
Plasmid#78743PurposeHuman expression plasmid for AsCpf1-NLS-3xHADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmammalian codon-optimized Cpf1-NLS-3xHA
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCAGAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330_DRS-1 sgRNA / hSpCas9
Plasmid#172841PurposeMammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
prCAG-MS2
Plasmid#191121PurposeTranscription of 47xCAG and 12xMS2 repeats from a tetracycline-inducible promoterDepositorInsertsrCAG
MS2
UseSynthetic BiologyPromoterTetAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-Cre-4x6T (AAV5)
Viral prep#196410-AAV5PurposeReady-to-use AAV5 particles produced from AAV-GfaABC1D-Cre-4x6T (#196410). In addition to the viral particles, you will also receive purified AAV-GfaABC1D-Cre-4x6T plasmid DNA. GfaABC1D driven expression of Cre recombinase in astrocytes. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAG and GfaABC1DAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TLR targeting vector
Plasmid#64215PurposeTargeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) constructDepositorInserttraffic light reporter (TLR) (AAVS1 Human)
UseHuman targetingMutationsee publication for detailsPromoterCAGAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by