We narrowed to 13,665 results for: crispr cas9

1. pYPQ150

   Plasmid

   #69301

   Purpose

   Gateway entry vector with pcoCas9 (Plant codon optimized)

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pcoCas9 (plant codon-optimized)
   

   Use

   CRISPR

   Tags

   3XFLAG

   Mutation

   NLS fusion

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBECAb-apr

   Plasmid

   #122001

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PU6::unc-119_sgRNA

   Plasmid

   #46169

   Purpose

   unc-119 targeting sgRNA

   Depositor

   John Calarco

   Article

   Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

   Insert

   unc-119 targeting sgRNA (unc-119 Synthetic)
   

   Use

   CRISPR

   Promoter

   C. elegans U6 snRNA pol III promoter

   Available Since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPK2660

   Plasmid

   #70709

   Purpose

   Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVDM1001

   Plasmid

   #134660

   Purpose

   CRISPR delivery and repair template delivery vector

   Depositor

   Willem van Schaik

   Article

   de Maat et al FEMS Microbiol Lett. 2019 Nov 1;366(22).

   Insert

   CRISPR guide RNA array
   

   Expression

   Bacterial

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. DR274-eGFP sgRNA

   Plasmid

   #61051

   Purpose

   for generation of a eGFP specific sgRNA from a T7 promoter

   Depositor

   Filippo Del Bene

   Article

   Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

   Insert

   EGFP sgRNA
   

   Use

   CRISPR

   Promoter

   T7

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-sgRNA-Lib

   Plasmid

   #53121

   Purpose

   sgRNA expression construction in a 3rd generation lentiviral backbone

   Depositor

   Wensheng Wei

   Article

   Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGY53

   Plasmid

   #221709

   Purpose

   Empty Cas12a vector for multiplex gene editing in fungi

   Depositor

   Kyle Pomraning

   Article

   Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

   Type

   Empty backbone

   Use

   CRISPR; Aspergillus

   Expression

   Bacterial

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. SGL40C.EFS.dTomato

   Plasmid

   #89395

   Purpose

   Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with dTomato expression (EFS Promoter)

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEM-3XP3.RFP-tan

    Plasmid

    #83474

    Purpose

    RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

    Depositor

    Patricia Wittkopp

    Article

    Lamb et al Fly (Austin). 2016 Aug 5:1-12.

    Insert

    3XP3.RFP-tan
    

    Use

    CRISPR

    Expression

    Bacterial and Insect

    Promoter

    3XP3 eye-expression Promoter

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNW3

    Plasmid

    #53372

    Purpose

    Csy4 and Cas9 D10A nickase expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Csy4-T2A-Cas9n (D10A)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-T3-hCasD10A-pA

    Plasmid

    #51638

    Purpose

    Expresses Cas9-nickase in mammalian cells and zygotes.

    Depositor

    Wataru Fujii

    Article

    Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

    Insert

    Codon-optimized Cas9 D10A
    

    Use

    CRISPR

    Tags

    FLAG, NLS, and polyA

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pFH6_new

    Plasmid

    #105866

    Purpose

    more efficient version of pFH6; Subcloning of any sgRNA via BbsI site

    Depositor

    Andreas Weber

    Article

    Hahn et al Bio Protoc. 2017 Jul 5;7(13):e2384. doi:

    Insert

    U6-26p::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Subcloning

    Expression

    Plant

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330A_FokI-1x2

    Plasmid

    #63602

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL690

    Plasmid

    #47753

    Purpose

    Expresses dCas9-VP64 fusion

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB5191

    Plasmid

    #126911

    Purpose

    Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    X-4 and XII-5 gRNA
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEM-WingGFP-tan

    Plasmid

    #83473

    Purpose

    EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

    Depositor

    Patricia Wittkopp

    Article

    Lamb et al Fly (Austin). 2016 Aug 5:1-12.

    Insert

    WingGFP-tan
    

    Use

    CRISPR

    Expression

    Bacterial and Insect

    Promoter

    Hsp70 and enhancer from D. melanogaster yellow ge…

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330A_FokI-1x4

    Plasmid

    #63604

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti

    Plasmid

    #138318

    Purpose

    Lenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    SauCas9 GFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ159

    Plasmid

    #69308

    Purpose

    Gateway entry vector with hSpCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    hSpCas9D10A (Human codon optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Mutation

    D10A mutation; NLS fusion

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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