Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 7,122 results for: poly

Clear keywords
Showing: 4981 - 5000 of 7122 results

  1. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid
    #118586
    Purpose
    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.
    Depositor
    Radhakrishnan Padmanabhan
    Article
    Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.
    Insert
    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    Use
    Yeast-e. col shuttle vector prs424
    Mutation
    The conserved GDD in the NS5 polymerase gene muta…
    Available Since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    118586-plasmid-map-sequence-id-227283

    Enlarge

  7. PB-Neo-TetO-Cdk12_Nterm_FlagHa

    Plasmid
    #127179
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector
    Depositor
    Phil Sharp
    Article
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Piggybac transposase vector
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mammalian
    Promoter
    TetO Promoter
    Available Since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    127179-plasmid-map-sequence-id-247939

    Enlarge

  10. Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

    Plasmid
    #208976
    Purpose
    Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    nSpCas9(H840)-BPNLS-P2A-GFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    208976-plasmid-map-sequence-id-415837

    Enlarge

  13. pCJ207

    Plasmid
    #162680
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162680-plasmid-map-sequence-id-321455

    Enlarge

Showing: 4981 - 5000 of 7122 results