We narrowed to 59,537 results for: cloning

1. pFH79

   Plasmid

   #125818

   Purpose

   Level0 A3A-PBE, wheat codon optimized

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   A3A-PBE, wheat codon optimized
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MXS_EF1a

   Plasmid

   #62421

   Purpose

   MXS_chaining vector with EF1a

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   EF1a Promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. GGE025

   Plasmid

   #120768

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_SynthGuo)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   T1_SynthGuo
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFH84

   Plasmid

   #128195

   Purpose

   Level1 ZmUBIp::A3A-PBE (wheat codon optimized):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::A3A-PBE (wheat codon optimized):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GGE258

   Plasmid

   #120775

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 3 (T3_Xpr2)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   T3_Xpr2
   

   Use

   Cloning/assembly plasmid

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH68

   Plasmid

   #128188

   Purpose

   Level1 ZmUBIp::SpCas9-NG (human codon optimized):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::SpCas9-NG (human codon optimized):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFH82

   Plasmid

   #128193

   Purpose

   Level1 ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFH93

   Plasmid

   #128196

   Purpose

   Level1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFH59

   Plasmid

   #128185

   Purpose

   Level1 ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFH81

    Plasmid

    #128192

    Purpose

    Level1 ZmUBIp::ScCas9 (wheat codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::ScCas9 (wheat codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GRM1

    Plasmid

    #25353

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GRM1-C254S:L28-G518 (GRM1 Human)
    

    Tags

    His

    Expression

    Insect

    Mutation

    C254S. Contains amino acids L28-G518.

    Available Since

    July 29, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

12. MXS_PGKenhancer

    Plasmid

    #62416

    Purpose

    MXS_chaining vector with PGKenhancer

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    PGK enhancer
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. ARL5:Q15-L176 (2H17)

    Plasmid

    #25527

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ARL5:Q15-L176 (ARL5A Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids Q15-L176

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pcDNA3.1 Flag YFP hNKCC1 A734C (NT508)

    Plasmid

    #50888

    Purpose

    Expresses human NKCC1 A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    A734C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUSC9

    Plasmid

    #50429

    Purpose

    Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 9.5 RVDs long.

    Depositor

    Andrew Scharenberg

    Article

    Boissel et al Nucleic Acids Res. 2013 Nov 26.

    Type

    Empty backbone

    Available Since

    Jan. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3E NWASP-CA

    Plasmid

    #105968

    Purpose

    3' element for Gateway cloning, NWASP C terminal domain

    Depositor

    Caren Norden

    Article

    Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

    Insert

    NWASP-CA
    

    Expression

    Bacterial

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MXS_PEST2D

    Plasmid

    #62434

    Purpose

    MXS_chaining vector with PEST2D

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Destabilization tag
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFOSGE_CUS1E04

    Plasmid

    #71076

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Sirt4 (Sirt4 Fly)
    

    Use

    Gateway entry vector

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pRTVIR

    Plasmid

    #46365

    Depositor

    Claes Andréasson

    Article

    Andreasson et al PLoS One. 2013 Sep 13;8(9):e74207. doi:

    Type

    Empty backbone

    Available Since

    July 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. SDCCAG10

    Plasmid

    #25610

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SDCCAG10 (CWC27 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Cyclophilin_CeCYP16-Like Domain (residues 8-173)

    Available Since

    Aug. 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits