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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,793 results for: T7

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Showing: 5021 - 5040 of 10793 results

  1. His-DERA

    Plasmid
    #203225
    Purpose
    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    deoxyribose aldolase
    Tags
    N-terminal TEV cleavable 6xHis tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203225-plasmid-map-sequence-id-400074

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  8. pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid
    #116955
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    116955-plasmid-map-sequence-id-226973

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  12. pcDNA3-Dronpa-Kras

    Plasmid
    #182329
    Purpose
    Plasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    Dronpa-Kras
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    182329-plasmid-map-sequence-id-363283

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  13. pCoofy48

    Plasmid
    #55186
    Purpose
    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    55186-plasmid-map-sequence-id-464535

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Showing: 5021 - 5040 of 10793 results