We narrowed to 12,019 results for: pcdna

1. pcDNA5/FRT/TO HIS DNAJB2b

   Plasmid

   #19551

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB2b
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4/TO-mu24-ORF24-2xStrep

   Plasmid

   #138453

   Purpose

   Expresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   mu24-ORF24 (ORF24 MHV68/KSHV)
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   mu24 amino acids 1-191 fused to ORF24 amino acids…

   Promoter

   CMV

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-Dest53-SHP-1 S591D

   Plasmid

   #21803

   Depositor

   Stephen Shaw

   Article

   Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

   Insert

   SHP-1 (PTPN6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Serine 591 to Aspartic acid

   Promoter

   Available Since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA-h-mmPtch1-B-NNQ-HA-GFP

   Plasmid

   #121164

   Purpose

   Expresses mmPtch1-B NNQ variant

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Ptch1 (Ptch1 Mouse)
   

   Use

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   deleted amino acids 620-643, truncated at 1291, N…

   Promoter

   CMV

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-h-mmPtch1-B-I766FV111FL114F-HA-GFP

   Plasmid

   #120909

   Purpose

   Expresses Ptch1-B (I766FV111FL114F)

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Ptch1 (Ptch1 Mouse)
   

   Use

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   deleted amino acids 620-643, truncated at 1291

   Promoter

   CMV

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-FLAG-Fos Y345A

   Plasmid

   #8970

   Depositor

   John Blenis

   Article

   Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

   Insert

   c-Fos (Fos Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y345A

   Promoter

   Available Since

   Sept. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-FLAG-RIPK3 4KR

   Plasmid

   #78821

   Purpose

   To overexpress RIPK3 4KR in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK3 (RIPK3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K55R, K89R, K363R, K501R

   Promoter

   CMV

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-TTF-1-Q50K-Y54M

   Plasmid

   #75264

   Purpose

   Expresses a double point mutant of human TTF-1

   Depositor

   David Mu

   Article

   Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

   Insert

   TTF-1 (NKX2-1 Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   Q50K and Y54M in the homeodomain

   Promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5/FRT/TO-Flag-Tipin

   Plasmid

   #23117

   Depositor

   Aziz Sancar

   Article

   Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

   Insert

   Tipin (TIPIN Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pcDNAmyc-hPR-B-486-933

    Plasmid

    #89118

    Purpose

    mammalian expression of human progesterone receptor amino acid residues 486-933 with N-terminal myc tag, has PR DNA and ligand binding domains

    Depositor

    Elizabeth Wilson

    Article

    Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

    Insert

    human progesterone receptor ligand binding domain (PGR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    contains aa 486-933

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-mCherry-CyrCR1.miR17

    Plasmid

    #128748

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-17 site inserted in mCherry 3'UTR.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with miR-17 site replacing miR-7 site (OIP5-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-RLuc8C-FHA1-NES

    Plasmid

    #138210

    Purpose

    Encodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    RLuc8C-FHA1-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3441 pcDNA3 Bid G60A

    Plasmid

    #8775

    Depositor

    Stanley Korsmeyer

    Article

    Zha et al Science 2000 Dec 1;290(5497):1761-5.

    Insert

    Bid p22 G60A (Bid Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G60A

    Promoter

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA5/FRT/TO HIS DNAK

    Plasmid

    #19544

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    dnaK molecular chaperone DnaK
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5 rtTA BirA eIF4G

    Plasmid

    #158789

    Purpose

    pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioID

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1
    

    Use

    Tags

    3xFlag and BirA*

    Expression

    Mammalian

    Mutation

    Promoter

    miniCMV

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-Flag-Med25-VWA

    Plasmid

    #64772

    Purpose

    Mammalian expression of Flag-tagged Med25-VWA

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25-VWA (MED25 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C-terminal deletion

    Promoter

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-FLAG-Fos F343A

    Plasmid

    #8969

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F343A

    Promoter

    Available Since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA-Dest53-SHP-1 S591A

    Plasmid

    #21802

    Depositor

    Stephen Shaw

    Article

    Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

    Insert

    SHP-1 (PTPN6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed Serine 591 to Alanine

    Promoter

    Available Since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pCDNA3.0_nucLAMA-G97 IRES mYFP

    Plasmid

    #130713

    Purpose

    Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-G97-NLS IRES mYFP
    

    Use

    Tags

    NLS and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-FLAG-RIPK1 K115R

    Plasmid

    #78843

    Purpose

    To overexpress RIPK1 K115R in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K115R

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge