166,012 results

1. pnCas9-AID

   Plasmid

   #196242

   Purpose

   Plasmid expressing the nCas9-AID fusion

   Depositor

   Andrea Puhar

   Article

   Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

   Insert

   nickase Cas9 fused to AID
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut4x

   Plasmid

   #182256

   Purpose

   Human PDAP1 3` UTR region mutated in 4x binding site for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of PDAP1 gene mutated in 4 binding sites for mir-150
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. SLP2a-TIR-GFP

   Plasmid

   #52738

   Purpose

   Retroviral vector expresses the lipid binding domain of SLP2a fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker

   Depositor

   Jonathan Kagan

   Article

   Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

   Insert

   Toll/IL-1 Receptor adaptor protein (TIRAP Human)
   

   Use

   Retroviral

   Tags

   C2A domain of Murine SLP2a and GFP

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-85

   Promoter

   Available Since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-PE2

   Plasmid

   #132775

   Purpose

   Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   PE2
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   CMV

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (AAV9)

   Viral Prep

   #124603-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (#124603). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 plasmid DNA. EF1a-driven, Cre-dependent expression of ChrimsonR fused to mRuby2 fluorophore and targeted to neuronal soma for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Christopher Harvey

   Article

   Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

   Promoter

   Tags

   mRuby2 (Cre-dependent)

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

6. pCDH-kz-CD20-Puro

   Plasmid

   #209759

   Purpose

   Lentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMD2.G-K47Q/R354Q

   Plasmid

   #207323

   Purpose

   VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   VSV-G (VSIVgp4 Vesicular Stomatitis Virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47Q/R354Q

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLS-SceI

   Plasmid

   #137725

   Purpose

   Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

   Depositor

   Nadav Ahituv

   Article

   Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1 hygro

   Plasmid

   #24150

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.

   Depositor

   Bob Weinberg

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA

   Available Since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHN924

    Plasmid

    #178885

    Purpose

    Expression of GST-His8-HRV3C

    Depositor

    Kazuhiro Abe

    Insert

    human rhinovirus 3C protease
    

    Use

    Tags

    GST, His8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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