Addgene

We narrowed to 23,361 results for: Nov;

Showing: 7561 - 7590 of 23361 results

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pSc-His6-Sem1Sc

Plasmid

#83235

Purpose

pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

Sem 1 (SEM1 Budding Yeast)

Use

Tags

His6

Expression

Yeast

Mutation

Promoter

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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NAR4-CD4d3+4-bio

Plasmid

#73118

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding NAR4 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

NAR4 (ART4 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pelB-MBP-BB0323-ss

Plasmid

#137067

Purpose

E. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66700.1

Use

Tags

PelB signal peptide + His10 + maltose binding pro…

Expression

Bacterial

Mutation

mature BB0323: lacks the BB0323 signal peptide

Promoter

T7lac

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCS3-MT-BX/p14ARF

Plasmid

#78761

Purpose

To overexpress p14ARF in Mammalian Cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

ARF (CDKN2A Human)

Use

Tags

6xMYC

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
ERMAP-CD4d3+4-bio

Plasmid

#73107

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding ERMAP with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ERMAP (ERMAP Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 VAMP2 R125TAG

Plasmid

#69876

Purpose

express in mammalian cells for unnatural amino acid incorporation into VAMP2-GFP

Depositor

Silvio Rizzoli

Article

Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

Insert

VAMP2-GFP (Vamp2 Rat)

Use

Synthetic Biology

Tags

GFP

Expression

Mammalian

Mutation

Amber stop codon in the linker between VAMP2 and …

Promoter

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
EF1a_MYC Ser62Ala_P2A_Hygro_Barcode

Plasmid

#120514

Purpose

Barcoded lentiviral vector to express MYC Ser62Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC Ser62Ala (MYC Human)

Use

Lentiviral

Tags

Expression

Mutation

Point mutation changing Serine to Alanine at amin…

Promoter

EF1a

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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CIAR_pcDNA5/FRT/TO

Plasmid

#86502

Purpose

Expresses CIAR in mammalian cells. Used to generate Flp-In stables.

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

CIAR (SOS1 Human)

Use

Frt

Tags

Expression

Mammalian

Mutation

T968L in SOScat

Promoter

CMV (tet operator)

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF11_P2A_Hygro_Barcode

Plasmid

#120497

Purpose

Barcoded lentiviral vector to express KLF11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF11 (KLF11 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Galectin3-CD4d3+4-bio

Plasmid

#73128

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Galectin3 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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AMIGO2-CD4d3+4-bio

Plasmid

#73103

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding AMIGO2 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

AMIGO2 (AMIGO2 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCDF1-ADAMTS18 Wt

Plasmid

#29552

Depositor

Yardena Samuels

Article

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Wild type

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
Lutheran-CD4d3+4-bio

Plasmid

#73101

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Lutheran with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Lutheran (BCAM Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAP79-AKAR4

Plasmid

#181957

Purpose

Genetically encoded FRET-based PKA activity reporter fused to AKAP79.

Depositor

Jin Zhang

Article

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

AKAP79-AKAR4 (AKAP5 Human)

Use

Tags

AKAP79, Cerulean, HIS tag, and cpVenus(E172)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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C1orf9-CD4d3+4-bio

Plasmid

#73109

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding C1orf9 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

C1orf9 (C1ORF9 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa1 AS

Plasmid

#69825

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCcnb1

Plasmid

#162793

Purpose

Ccnb1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Ccnb1 shRNA (Ccnb1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CreLite

Plasmid

#131785

Purpose

AAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoter

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC-P2A-PIF6CreN

Use

AAV, Cre/Lox, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Chicken ArcLight Q175

Plasmid

#53566

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Chicken ArcLight-Q175

Use

Tags

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-iC++

Plasmid

#98165

Purpose

artificial anion conducting channelrhodopsin (aACR). Activation max 488 nm; off-kinetics 35 ms. Codon optimized for mammalian expression.

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct iC++ gene

Use

Tags

mCherry

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, V242R, T24…

Promoter

CMV (+enhancer)

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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DSCAML1-CD4d3+4-bio

Plasmid

#73105

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding DSCAML1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

DSCAML1 (DSCAML1 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp1

Plasmid

#157680

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-Puro-SIK2

Plasmid

#138696

Purpose

Expresses a human SIK2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK2 human (SIK2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-SIK3

Plasmid

#138697

Purpose

Expresses a human SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 human (SIK3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-FUSN-miRFP670-Cry2WT

Plasmid

#122441

Purpose

Expresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pBW_0001

Plasmid

#102833

Purpose

Binary vector containing Sr22 Schomburgk allele driven by maize ubiquitin promoter, Sr22 terminator

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

Sr22 Schomburgk (NEWENTRY Synthetic)

Use

Tags

Expression

Plant

Mutation

Promoter

Maize ubiquitin

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Bmi1

Plasmid

#61532

Purpose

Expresses Bmi1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Bmi1 (Bmi1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pRD408

Plasmid

#178199

Purpose

Expression of H-NS from its natural promoter

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

phns + hns (hns Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

hns promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGW045 AAV-CRISPRa base vector pAAV_OE-U6sgbbSapI(MS2)-EF1a-MPH-WPRE

Plasmid

#192161

Purpose

AAV-CRISPRa base vector

Depositor

Sidi Chen

Article

Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(WT)-IRES-Neo

Plasmid

#128663

Purpose

mammalian expression of Flag-Polß(WT) protein with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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