We narrowed to 14,090 results for: cas9

1. pRU41

   Plasmid

   #167663

   Purpose

   ENTRY vector for gRNA1 cloning under pAtU6 promoter

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pAtU6
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRU42

   Plasmid

   #167664

   Purpose

   ENTRY vector for gRNA2 cloning under pAtU3 promoter

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pAtU3
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRU43

   Plasmid

   #167665

   Purpose

   ENTRY vector for gRNA3 cloning under pAtU6 promoter

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pAtU6
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6a:sgRNA#1

   Plasmid

   #64245

   Purpose

   expresses sgRNA under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-SEP-GluA2 TKIT

   Plasmid

   #169442

   Purpose

   Expression of 2 guides + donor DNA

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   AAV and CRISPR

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX-sgRNA

   Plasmid

   #50662

   Purpose

   Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   sgAAVS1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDD104 (Peft-3::Cre)

   Plasmid

   #47551

   Purpose

   Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Insert

   Cre Recombinase
   

   Use

   Cre/Lox

   Expression

   Worm

   Promoter

   eef-1A.1 (eft-3)

   Available Since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX856

   Plasmid

   #62888

   Purpose

   C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa536-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS, NLS SV40, and VP64

   Expression

   Mammalian

   Mutation

   Asparagine 863 to Alanine (N863A)

   Promoter

   CBh

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX855

   Plasmid

   #62887

   Purpose

   N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa 2-535)
   

   Use

   CRISPR

   Tags

   FRB and NES PTK2

   Expression

   Mammalian

   Mutation

   Aspartic acide 10 to Alanine (D10A)

   Promoter

   CBh

   Available Since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMiniT:SEP-GluA2 TKIT donor

    Plasmid

    #169421

    Purpose

    Donor DNA for TKIT knockin of SEP to GluA2 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYZ463

    Plasmid

    #187971

    Purpose

    TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6b:sgRNA#3

    Plasmid

    #64247

    Purpose

    expresses sgRNA under U6b promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Tet-inducible mCherry reporter

    Plasmid

    #64128

    Purpose

    Photoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoter

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    mCherry
    

    Expression

    Mammalian

    Promoter

    TetOx13-CMV

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6c:sgRNA#4

    Plasmid

    #64248

    Purpose

    expresses sgRNA under U6c promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMiniT:SEP-GluN2A TKIT donor

    Plasmid

    #169429

    Purpose

    Donor DNA for TKIT knockin of SEP to NR2A in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMiniT:SEP-GluN2B TKIT donor

    Plasmid

    #169431

    Purpose

    Donor DNA for TKIT knockin of SEP to NR2B in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMiniT:SEP-GluN1 TKIT donor

    Plasmid

    #169427

    Purpose

    Donor DNA for TKIT knockin of SEP to NR1 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6a:sgRNA#2

    Plasmid

    #64246

    Purpose

    expresses sgRNA under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHFS:SEP-rGluA1 TKIT donor

    Plasmid

    #169438

    Purpose

    Donor DNA for TKIT knockin of SEP to GluA1 in rat

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6d:sgRNA#5

    Plasmid

    #64249

    Purpose

    expresses sgRNA under U6d promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge