We narrowed to 7,663 results for: ski

1. pENTR-myr-AKT-HA

   Plasmid

   #31790

   Depositor

   Xin Chen

   Article

   Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Entry vector

   Tags

   HA and Myr

   Available Since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

2. His-FLAG-Tev_ACTA1 (alpha-actin)

   Plasmid

   #188452

   Purpose

   Express in mammalian cells human alpha-actin (ACTA1) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

   Depositor

   Roberto Dominguez

   Article

   Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

   Insert

   ACTA1 (ACTA1 Human)
   

   Tags

   6xHis-FLAG

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-EF1a-IRES-Puro PFKM

   Plasmid

   #201638

   Purpose

   Over-expression of untagged human PFKM

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   PFKM (PFKM Human)
   

   Use

   Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TurboID-13x-EV

   Plasmid

   #215086

   Purpose

   Expresses TurboID-13x[linker] in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   TurboID (birA )
   

   Use

   Retroviral

   Tags

   13x [linker]

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PL-5LTR-GW-A

   Plasmid

   #138480

   Purpose

   For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
   

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-FLAG-GRM3-VN

   Plasmid

   #98965

   Purpose

   FLAG-tagged human mGluR3 fused to N-terminus of split Venus

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   GRM3 (GRM3 Human)
   

   Tags

   FLAG (dykddddk) epitope tag, Signal/leader sequen…

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT-TO-Nsp13_GC3opt

   Plasmid

   #157714

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Available Since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pET21a-C2m2-mKate

   Plasmid

   #208765

   Purpose

   To express C2m2-mKate in bacteria

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-mKate (Mfge8 Mouse)
   

   Tags

   mKate

   Expression

   Bacterial

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   T7 promoter

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. YA1532: pAAV_ CAG-FLEX-paQuasAr3

   Plasmid

   #107702

   Purpose

   in vivo voltage imaging

   Depositor

   Adam Cohen

   Article

   Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

   Insert

   paQuasAr3
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD2sE_SC14

    Plasmid

    #219717

    Purpose

    DV2 stable E dimer

    Depositor

    Brian Kuhlman

    Article

    Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

    Insert

    DV2 sE SC14
    

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    A259W, T262R, F279W, T280P

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-MYO10-HMM

    Plasmid

    #87256

    Purpose

    Expression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Expression

    Mammalian

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNL-CEF-IgM-IRES-GFP

    Plasmid

    #187011

    Purpose

    Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM

    Depositor

    Hermann Eibel

    Article

    Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

    Insert

    Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM
    

    Use

    Lentiviral

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUW-TetO-Cdx2

    Plasmid

    #128829

    Purpose

    dox-dependent expression of mouse Cdx2

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Cdx2 (Cdx2 Mouse)
    

    Use

    Lentiviral

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETDuet-1 Flag-LplA

    Plasmid

    #232118

    Purpose

    For bacterial expression of Flag-tagged E. coli LplA.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    lplA (lplA Escherichia coli (strain K12))
    

    Tags

    Flag

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1/NDUFV2/myc-His

    Plasmid

    #127491

    Purpose

    Expresses myc-tagged NDUFV2 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFV2 (NDUFV2 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

    Plasmid

    #106204

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    GltI: A184S + mRuby3 fusion

    Promoter

    CAG

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIBN-tagBFP-hTRF2

    Plasmid

    #103803

    Purpose

    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    hTRF2: deletion of amino acids 1-42 and 476 compa…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX FynA-SH2

    Plasmid

    #46429

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    FynA (FYN Human)
    

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 142-251

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

    Plasmid

    #119723

    Purpose

    AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Thomas Oertner

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Inserts

    CaMPARI2
    CaMPARI2
    

    Use

    AAV

    Tags

    NES-his

    Expression

    Mammalian

    Mutation

    F391W, L398V

    Promoter

    hSyn1

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNL-CEF-IgG1-IRES-GFP

    Plasmid

    #187008

    Purpose

    Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1

    Depositor

    Hermann Eibel

    Article

    Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

    Insert

    Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1
    

    Use

    Lentiviral

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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