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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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172,720 results

Showing: 5081 - 5100 of 172720 results

  2. MoClo Yeast Secretion and Display (YSD) Toolkit

    Plasmid Kit
    #1000000240
    Purpose
    Collection of plasmids to optimize secretion and cell surface display of proteins of interest in yeast.
    Depositor
    Paul Young
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  6. InsRA-eGFP

    Plasmid
    #79795
    Purpose
    Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
    Depositor
    James Johnson
    Article
    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:
    Insert
    InsRA (INSR Human)
    Tags
    eGFP extracellular
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    79795-plasmid-map-sequence-id-149406

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  7. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid
    #227274
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227274-plasmid-map-sequence-id-452035

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  9. pTFE3.1.0-gDNA

    Plasmid
    #112473
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFE3
    Depositor
    Kevin White
    Insert
    TFE3 (TFE3 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112473-plasmid-map-sequence-id-230504

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  10. mycBioID2-13X Linker-MCS

    Plasmid
    #92308
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    Humanized A. aeolicus BioID2 with R40G and Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    92308-plasmid-map-sequence-id-216421

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  17. pB025

    Plasmid
    #183092
    Purpose
    Expresses FnCas12a in Bacteroides and used for genome editing
    Depositor
    Lei Dai
    Article
    Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47
    Insert
    FnCas12a, gRNA, Promoter, HAB, TetR,
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P1TDPGH023
    Available Since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183092-plasmid-map-sequence-id-368147

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Showing: 5081 - 5100 of 172720 results