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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,110 results for: NSI

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Showing: 10161 - 10200 of 12110 results

  5. Nabi2.242

    Plasmid
    #73754
    Purpose
    Nabi2.242 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.
    Depositor
    Bradley Baker
    Article
    Sung et al PLoS One. 2015 Nov 20;10(11):e0141585. d
    Insert
    Nabi2.242
    Expression
    Mammalian
    Available Since
    April 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73754-plasmid-map-sequence-id-136686

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  8. BC-A1-014

    Plasmid
    #78702
    Purpose
    A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
    Use
    Synthetic Biology
    Available Since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78702-plasmid-map-sequence-id-171059

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  17. GFP-X3

    Plasmid
    #115568
    Purpose
    Synthetic acetylation substrate consisting of GFP fusion with 3 repeats of the Gcn5 consensus motif
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    Green Fluorescent Protein
    Expression
    Bacterial and Yeast
    Mutation
    Three repeats of the Gcn5 consensus motif are tag…
    Promoter
    ADH1
    Available Since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    115568-plasmid-map-sequence-id-226342

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  18. BC-A1-011

    Plasmid
    #78699
    Purpose
    A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
    Use
    Synthetic Biology
    Available Since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78699-plasmid-map-sequence-id-171057

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  19. BC-A1-009

    Plasmid
    #78696
    Purpose
    A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
    Use
    Synthetic Biology
    Available Since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78696-plasmid-map-sequence-id-171054

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  22. pJT3

    Plasmid
    #98304
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of CUP1 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.
    Insert
    TRPL3AcNES1>TRPL41B
    Expression
    Yeast
    Available Since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98304-plasmid-map-sequence-id-190175

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  24. pCALNL_Ch10

    Plasmid
    #81224
    Purpose
    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in PCDH15 locus on chromosome 10
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    gix-Neo-gix deletion cassette upstream of EGFP
    Expression
    Mammalian
    Promoter
    pCBa
    Available Since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    81224-plasmid-map-sequence-id-163294

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  28. pXeDNA3.1-HsTRPV1

    Plasmid
    #244454
    Purpose
    pXeDNA3.1-HsTRPV1 encodes human TRPV1 in CMV/Xenopus β‑globin UTR scaffold for robust expression in mammalian cells and Xenopus oocytes; BGH polyA, AmpR, NeoR, lacZα for blue‑white screening.
    Depositor
    Luis Yanez-Guerra
    Insert
    Transient receptor potential cation channel subfamily V member 1 (TRPV1 Human)
    Use
    Xenopus oocyte expression
    Expression
    Mammalian
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    244454-plasmid-map-sequence-id-490929

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  32. ACE2-Mutant-R652A

    Plasmid
    #240605
    Purpose
    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2; R was substituted by A at Residue 652 of ACE2 in the collectrin-like site
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Arginine (R) at position 652 on ACE2 is replaced …
    Promoter
    CMV
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    240605-plasmid-map-sequence-id-483848

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  33. ACE2-Mutant-C1

    Plasmid
    #240604
    Purpose
    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 1 located within the collectrin-like site on ACE2
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Arginine (R) and Lysine (K) residues on ACE2-clus…
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240604-plasmid-map-sequence-id-482832

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  34. ACE2-Mutant-DeltaC4

    Plasmid
    #240609
    Purpose
    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2 likely at Cluster 4 of ACE2 in the collectrin-like site. The whole C4 sequence was deleted in this mutant
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    deletion of the full Cluster 4 sequence on ACE2 (…
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240609-plasmid-map-sequence-id-482819

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  35. ACE2-Mutant-C0

    Plasmid
    #240603
    Purpose
    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 0 located within the collectrin-like site on ACE2
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Arginine (R) and Lysine (K) residues on ACE2-clus…
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240603-plasmid-map-sequence-id-482814

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  36. ACE2-Mutant-C3

    Plasmid
    #240607
    Purpose
    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 3 located within the collectrin-like site on ACE2
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Arginine (R) and Lysine (K) residues on ACE2-clus…
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240607-plasmid-map-sequence-id-482813

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  38. pRSF_AfRbcLS

    Plasmid
    #229518
    Purpose
    Expresses Anthoceros fusiformis Rubisco large and small subunit (C terminus hexahistidine)
    Depositor
    Laura Gunn
    Article
    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.
    Inserts
    Rubisco large subunit
    Rubisco small subunit
    Tags
    hexahistidine
    Expression
    Bacterial
    Mutation
    N terminus chloroplast transit peptide truncation
    Promoter
    T7
    Available Since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229518-plasmid-map-sequence-id-456759

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  39. pRSF_PcRbcLS

    Plasmid
    #229519
    Purpose
    Expresses Phaeoceros carolinianus Rubisco large and small subunit (C terminus hexahistidine)
    Depositor
    Laura Gunn
    Article
    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.
    Inserts
    Rubisco large subunit
    Rubisco small subunit
    Tags
    hexahistidine
    Expression
    Bacterial
    Mutation
    N terminus chloroplast transit peptide truncation
    Promoter
    T7
    Available Since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229519-plasmid-map-sequence-id-457204

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  40. pRSF_NoRbcLS

    Plasmid
    #229520
    Purpose
    Expresses Notothylas orbicularis Rubisco large and small subunit (C terminus hexahistidine)
    Depositor
    Laura Gunn
    Article
    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.
    Inserts
    Rubisco large subunit
    Rubisco small subunit
    Tags
    hexahistidine
    Expression
    Bacterial
    Mutation
    N terminus chloroplast transit peptide truncation
    Promoter
    T7
    Available Since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229520-plasmid-map-sequence-id-457193

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Showing: 10161 - 10200 of 12110 results