We narrowed to 18,038 results for: Mut

1. pLentiCrispr-V2-mOrange (V2mO)

   Plasmid

   #140206

   Purpose

   Lenti Crispr/Cas9 with mOrange fluorescent marker

   Depositor

   Jaffer Ajani

   Article

   Jin et al PLoS One. 2020 Feb 13;15(2):e0228910. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pELF1a

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-mitoTPNOX

   Plasmid

   #87854

   Purpose

   This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

   Insert

   mitoTPNOX
   

   Use

   Tags

   C-terminal FLAG tag with a linker and mitochondri…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-hNM2B

   Plasmid

   #236533

   Purpose

   For expression of NM2B (human) in mammalian cells

   Depositor

   Sandra Citi

   Article

   Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

   Insert

   MYH10 (MYH10 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPolI_SARS-CoV-2_NLuc

   Plasmid

   #183953

   Purpose

   Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

   Depositor

   AJ te Velthuis

   Article

   Vial et al bioRxiv 2021.06.28.450211

   Insert

   SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mammalian Pol I

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac-CHD7

   Plasmid

   #170141

   Purpose

   Expresses CHD7 in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCold TDP43 WT

   Plasmid

   #27451

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   Factor Xa, GST, and His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-alphasynuclein-A53T

   Plasmid

   #40823

   Depositor

   David Rubinsztein

   Article

   Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

   Insert

   SNCA (SNCA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   A53T (aggregates more rapidly than the wild-type …

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEX-EGFP-KASH

   Plasmid

   #154373

   Purpose

   Vector for Cre-dependent expression of EGFP tagged KASH protein domain

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   EGFP-KASH
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. FKBP-CD28TMD-NTEVp(H75S)

   Plasmid

   #137828

   Purpose

   MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-CD28TMD-NTEVp(H75S)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Mutated Histidine 75 to Serine in NTEVp fragment

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-28b-Cas9-His

    Plasmid

    #47327

    Purpose

    For in vitro expression and purification of Cas9 protein

    Depositor

    Alex Schier

    Article

    Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMax_GFP

    Plasmid

    #177825

    Purpose

    Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone)

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9n(BB)-2A-GFP (PX461)

    Plasmid

    #48140

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLIB-HIS-TEV-scCAK1

    Plasmid

    #177014

    Purpose

    Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
    

    Use

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEXfrt-SaCas9-U6-sgRNA

    Plasmid

    #124845

    Purpose

    Vector for FLP-dependent expression of SaCas9 with gRNA

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SauCas9 gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSN837

    Plasmid

    #184825

    Purpose

    Expresses human KIF5A in mammalian cells

    Depositor

    Kyoko Chiba

    Article

    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

    Insert

    KIF5A (KIF5A Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-ExRai-AMPKAR(T/A)

    Plasmid

    #192447

    Purpose

    Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR(T/A)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Thr 6 in AMPK substrate mutated to Ala.

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid

    #62987

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pH2rU3_ForInd_mCherry_CMV_ZsGT2APurR

    Plasmid

    #204579

    Purpose

    lentivirus backbone cloning vector and template for barcoded non-neutralizable standard production

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AMPK(T>A)-SPARK

    Plasmid

    #226256

    Purpose

    phospho-null mutant of AMPK-SPARK

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    AMPK(T>A)-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    substituted Threonine 20 with Alanine

    Promoter

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PIK3CA-WT

    Plasmid

    #16643

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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