164,811 results

1. pMRE-Tn7-156

   Plasmid

   #118570

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mCardinal
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYX233-DHFR

   Plasmid

   #163761

   Purpose

   Gal-inducible yeast expression of cytosolic DHFR

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   DHFR (Dhfr Mouse)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pL2Cas9

   Plasmid

   #98841

   Purpose

   Genome engineering of virulent phages

   Depositor

   Sylvain Moineau

   Article

   Lemay et al ACS Synth Biol. 2017 Mar 30. doi: 10.102

   Insert

   tracr/Cas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. C321.∆A ompT- rne- endA- lon-

   Bacterial Strain

   #182775

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Ampicillin

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

5. MBP-CHC(1-1074)-His6

   Plasmid

   #100748

   Purpose

   Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CLTC (CLTC Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Residues 1-1074

   Promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PAGFP-Ub

   Plasmid

   #11933

   Depositor

   Nico Dantuma

   Article

   Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

   Insert

   Ubiquitin (RBX1 Human)
   

   Use

   Tags

   PAGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-MAO-N Mitochondrial Membrane-Targeting Cloning Vector

   Plasmid

   #139015

   Purpose

   To express a protein of interest fused to the N-terminus of HA-MAO for mitochondrial outer membrane targeting

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   HA-MAO

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti PGK Neo DEST (w531-1)

   Plasmid

   #19067

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Neo

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEFL EGFP-TEADi

   Plasmid

   #140144

   Purpose

   TEAD inhibitor (TEADi): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal.

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

   Insert

   EGFP TEADi
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-CMV-GCaMP6s-CAAX

    Plasmid

    #52228

    Purpose

    Plasma membrane targeted GCaMP6s, calcium sensor

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    GCaMP6s-CAAX
    

    Use

    Tags

    6xHis and CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. TALED_Left-ND1-AD-E1347A

    Plasmid

    #183894

    Purpose

    Expresses TALED_Left-ND1-AD-E1347A in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP_sfCherry2(11)_Clathrin light chain

    Plasmid

    #83032

    Purpose

    Expresses sfCherry2(11) tagged clathrin light chain in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_Clathrin light chain (CLTA Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-GFP (AAV CAP-B10)

    Viral Prep

    #37825-CAP-B10

    Purpose

    Ready-to-use AAV CAP-B10 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV were produced with the CAP-B10 serotype, which permits efficient transduction of both inhibitory and excitatory neurons in the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    GFP

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

14. NbV5-HiBiT

    Plasmid

    #201489

    Purpose

    NanoBit detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbV5-HiBiT
    

    Use

    Tags

    HiBit

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHEN-CoV2-nanobody-F1

    Plasmid

    #194592

    Purpose

    Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    F1 (S )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE (AAV8)

    Viral Prep

    #131004-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE (#131004). In addition to the viral particles, you will also receive purified pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE plasmid DNA. hSyn-driven expression of calcium sensor GCaMP6m and bicistronic ChRmine (red-shifted channelrhodopsin). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Promoter

    Syn

    Tags

    None

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

17. pX601-AAV-CMV-NLS-ISTfu1-NLS-3xHA-bGHpA;U6-BbsI-reRNA

    Plasmid

    #205319

    Purpose

    All-in-one AAV vector expressing ISTfu1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISTfu1 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-Flag-Med25

    Plasmid

    #64771

    Purpose

    Mammalian protein expression of flag-tagged Med25

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25 (MED25 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pZFN-AAVS1-KKR

    Plasmid

    #159298

    Purpose

    ZFN (right) targeting the AAVS1 locus with obligate heterodimer FokI

    Depositor

    Kosuke Yusa

    Insert

    ZFN targeting the AAVS1 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pM201

    Plasmid

    #173179

    Purpose

    dCas9-24xGCN4

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    dCas9-24xGCN4
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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