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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 36,290 results for: lat

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Showing: 5161 - 5180 of 36290 results

  1. pJW1354

    Plasmid
    #69490
    Purpose
    Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms
    Depositor
    Jordan Ward
    Publication
    Zhang et al Development. 2015 Nov 9. pii: dev.129635
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
    Use
    CRISPR; Template to generate cassettes for -media…
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69490-plasmid-map-sequence-id-122661

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  3. pFLAG_attP

    Plasmid
    #110095
    Purpose
    Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.
    Depositor
    Markus Seeger
    Publication
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Tags
    3xFLAG
    Expression
    Bacterial
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    110095-plasmid-map-sequence-id-317064

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  5. SNAP-OMP25

    Plasmid
    #141149
    Purpose
    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells
    Depositor
    Gia Voeltz
    Publication
    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OMP25 (Synj2bp Budding Yeast, Rat)
    Tags
    SNAP tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    141149-plasmid-map-sequence-id-309902

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  6. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

    Plasmid
    #126706
    Purpose
    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence
    Depositor
    Darrell Kotton
    Publication
    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
    Use
    CRISPR
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    126706-plasmid-map-sequence-id-245754

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  9. pCDH_Str-KDEL_ManII-SBP-mCherry

    Plasmid
    #65259
    Purpose
    synchronize trafficking of ManII from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Insert
    Streptavidin-KDEL and truncated Mannosidase II
    Use
    Lentiviral
    Tags
    Streptavidin Binding Protein (SBP) and mCherry
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65259-plasmid-map-sequence-id-120695

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  17. pcDNA3.1-GGamma7-GFP2

    Plasmid
    #166778
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGamma7-GFP2 (GNG7 Human, Synthetic)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    166778-plasmid-map-sequence-id-331001

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  19. PGL4-4X TE Down (GQs) Renilla

    Plasmid
    #70039
    Purpose
    Plasmid for testing 5’UTR TE Down (G-quadruplex) folding elements
    Depositor
    Hans-Guido Wendel
    Publication
    Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    4x TE Down (G-quadruplexes) in 5'UTR
    Use
    Luciferase
    Tags
    renilla luciferase
    Expression
    Mammalian
    Mutation
    4 repeats of TE down motif (G-quadruplexes) in 5&…
    Promoter
    SV40
    Available since
    Jan. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 5161 - 5180 of 36290 results