We narrowed to 41,904 results for: tro

1. UAS-u(S)Cas9

   Plasmid

   #127383

   Purpose

   Expresses Cas9 at high level

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   uORF(S)-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAST

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3xFLAG-dCas9/pMXs-IG

   Plasmid

   #51258

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZac2.1 U6-SaDMDR7-U6-SaDMDL2

   Plasmid

   #78603

   Purpose

   AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   U6-SaDMDR7-U6-SaDMDL2
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-FDIO-COX4-dAPEX2

   Plasmid

   #117178

   Purpose

   Plasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   COX4-dAPEX2
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLXSN-H-Ras_N17

   Plasmid

   #39537

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

   Insert

   HRAS N17 (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   N17

   Promoter

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pK1E-RNAP

   Plasmid

   #48144

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   rna polymerase K1E
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+ EB3-mKate2

   Plasmid

   #105940

   Purpose

   in vitro transcription

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   EB3 (MAPRE3 Human)
   

   Use

   Tags

   mKate2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry

   Plasmid

   #136603

   Purpose

   Encodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

   Depositor

   John Ngo

   Article

   Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

   Insert

   FKBP-C-mSOG-J-alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-RNF68

   Plasmid

   #126545

   Purpose

   Expresses mCherry-tagged RNF68 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

   Insert

   RNF68 (PCGF1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas9-VRER_2A_GFP

    Plasmid

    #75475

    Purpose

    Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

    Depositor

    Marc Tessier-Lavigne

    Article

    Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

    Insert

    Cas9-VRER
    

    Use

    CRISPR

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    CAG

    Available since

    May 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-SAM-hygro

    Plasmid

    #138955

    Purpose

    Lentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 fused to MS2 binding protein, and hygromycin resistance gene

    Depositor

    Nadya Dimitrova

    Article

    Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 AND EF1a

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSMP-EZH1_1

    Plasmid

    #36359

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    EZH1 (EZH1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pBABE MDER

    Plasmid

    #13494

    Depositor

    Stephen Tapscott

    Article

    Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90

    Insert

    MyoD1-Estrogen Receptor Fusion Protein (Myod1 Human, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    full length MyoD was fused to aa282-595 of Esr1. …

    Promoter

    Available since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHM123DEnv

    Plasmid

    #137668

    Purpose

    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

    Depositor

    Anjali Joshi

    Article

    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

    Insert

    HIV-1 Envelope Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWzyE-distrepII

    Plasmid

    #196667

    Purpose

    Expression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzyE
    

    Use

    Tags

    TEV-cleavable di-strep tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSMP-Bmi1_1

    Plasmid

    #36352

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    Bmi1 (BMI1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-HA-RNF68

    Plasmid

    #126548

    Purpose

    Expresses FLAG-HA-tagged RNF68 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF68 (PCGF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.Flag.HERC2-HECT-WT.6xHis

    Plasmid

    #39227

    Depositor

    Aziz Sancar

    Article

    Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

    Insert

    HERC2 HECT Domain (HERC2 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

19. mADAM10 E385A in pQCXIP cFLAG

    Plasmid

    #19137

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM10 (Adam10 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E385A

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pBMN YFP-ltDHFR(DD)

    Plasmid

    #47080

    Purpose

    ecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegans

    Depositor

    Thomas Wandless

    Article

    Cho et al PLoS One. 2013 Aug 22;8(8):e72393. doi:

    Insert

    YFP-ltDHFR(DD)
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    amino acids mutations: R12H, N37D, N59D, G67S, D1…

    Promoter

    LTR

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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