We narrowed to 4,533 results for: Gaa

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Bet1L-mCherry

Plasmid

#209849

Purpose

Expresses Bet1L-mCherry in mammalian cells

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

Bet1L (BET1L Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/GFP4_Seq1.3

Plasmid

#206136

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1 (Col1a1 Mouse)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FRB_T2098L-VAMP3-mCherry

Plasmid

#92429

Purpose

Expression of VAMP3 N-terminally conjugated to FRB and C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

VAMP3 (Vamp3 Mouse)

Tags

FKBP rapamycin binding (FRB) domain of mTOR with …

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-SNCA-A30P

Plasmid

#180016

Purpose

Transiently expressing a pegRNA to introduce SNCA-A30P mutation in human cells

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for SNCA-A30P (SNCA Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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shCCL20

Plasmid

#65096

Purpose

retroviral expression of CCL20 shRNA

Depositor

Pau Sancho-Bru

Article

Affo et al Gut. 2014 Nov;63(11):1782-92. doi: 10.11

Insert

Ccl20 shRNA (Ccl20 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
MAP4K3 gRNA (BRDN0001147653)

Plasmid

#76406

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP4K3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP4K3 (MAP4K3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-Rorb sesRNA-2a-msFlag-2a-tTA2-WPRE

Plasmid

#239029

Purpose

Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for mouse Rorb (Rorb Mouse)

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet_Seq1.3

Plasmid

#206137

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1 (Col1a1 Mouse)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shAtxn1

Plasmid

#180387

Purpose

Producing AAV that encodes mouse Atxn1 shRNA with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shAtxn1 (Atxn1 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-hPIKfyve

Plasmid

#121148

Purpose

Expresses GFP-tagged human PIKfyve in mammalian cells.

Depositor

Geert van den Bogaart

Article

Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

Insert

1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IDO2-p2a-eGFP

Plasmid

#187027

Purpose

Expresses IDO2 and GFP (seperately) in mammalian cells

Depositor

Geert van den Bogaart

Article

Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

Insert

Indoleamine 2,3-dioxygenase 2 (IDO2 Human)

Tags

P2A-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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IST1-GFP

Plasmid

#186298

Purpose

Expresses human IST1 C-terminally fused to GFP

Depositor

Geert van den Bogaart

Article

Stempels et al J Cell Biol. 2023 Jul 3;222(7):e20220513

Insert

IST1 homolog (IST1 Human)

Tags

GFP

Expression

Mammalian

Mutation

GFP

Promoter

CMV

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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peGFP-N1-Cofilin

Plasmid

#186747

Purpose

Expresses wildtype GFP-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Tags

GFP

Expression

Mammalian

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1-cofilin

Plasmid

#186750

Purpose

Expresses wildtype mCherry-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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peGFP-N1-Cofilin-S41E

Plasmid

#186748

Purpose

Expresses S41E phosphomimetic GFP-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed serine 41 to glutamic acid

Promoter

CMV

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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peGFP-N1-Cofilin-S41A

Plasmid

#186749

Purpose

Expresses S41A phosphodead GFP-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed serine 41 to alanine

Promoter

CMV

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1-Cofilin-S41E

Plasmid

#186751

Purpose

Expresses S41E phosphomimetic mCherry-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Changed serine 41 to glutamic acid

Promoter

CMV

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pT2-shATRX

Plasmid

#124258

Purpose

Expresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.

Depositor

Maria Castro

Article

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

shATRX

Expression

Mammalian

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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str-KDEL_Stx5LdeltaER-SBP-mCitrine

Plasmid

#154846

Purpose

Synchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system)

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

Stx5 (Long isoform) (STX5 Human)

Tags

Streptavidin Binding Protein (SBP) and mCitrine

Expression

Mammalian

Mutation

Changed Methionine 55 to Valine, causes loss of S…

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

Plasmid

#113156

Purpose

An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

Two gRNAs for rat Rosa26

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mU6 and hU6

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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