Addgene

We narrowed to 586 results for: beta-actin

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NDV-HN-SpyTag

Plasmid

#160000

Purpose

Expresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

NDV-HN-SpyTag

Use

Tags

His6-tag

Expression

Mammalian

Mutation

Promoter

Chicken beta-actin promoter

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-hWIPI1A-3×FLAG

Plasmid

#215509

Purpose

Expresses FLAG tagged human WIPI1A.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 1A (WIPI1 Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-TetON-3G

Plasmid

#96963

Purpose

Mammalian expression of Clontech rtTA driven by human beta-actin promoter

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGs-NLS-TIR1_P2A_NES-TIR1

Plasmid

#117699

Purpose

Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Inserts

TRANSPORT INHIBITOR RESPONSE 1
TRANSPORT INHIBITOR RESPONSE 1

Use

Tags

HA tag, Myc-NES, and NLS

Expression

Mammalian

Mutation

codon optimized for murine expression

Promoter

None, 2A peptide from porcine teschovirus-1 polyp…

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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2xFKBP-mCh-KDEL

Plasmid

#173010

Purpose

FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

2xFKBP-mCh-KDEL

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Chicken beta actin (CAG)

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027

Plasmid

#134611

Purpose

Mammalian expression of mouse KIF5A 378-1027

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5A (Kif5a Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 377 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCAX-EGFP-FBP17

Plasmid

#178365

Purpose

Expresses FBP17 c-terminal labeled with EGFP in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Formin Binding Protein 17 (FNBP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken Beta Actin

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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DHFR-mNeon-GluA1

Plasmid

#173009

Purpose

For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

DHFR-mNeon-GluA1

Use

Tags

DHFR and mNeon

Expression

Mammalian

Mutation

Promoter

Chicken beta actin (CAG)

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBa-flag-BicD2 594-FKBP

Plasmid

#64206

Purpose

Bicaudal D protein aa1-594 (excluding start Met) with N-terminal flag tag and C-terminal FKBP

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Bicaudal D protein (Bicd2 Mouse)

Use

Tags

FKBP and flag

Expression

Mammalian

Mutation

no start methionine, aa1-594

Promoter

chicken Beta Actin

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS mSynCAM1

Plasmid

#115496

Purpose

expression of mouse full-length SynCAM 1, CAG promoter suited for neuronal expression

Depositor

Thomas Biederer

Article

Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

Insert

Cadm1 (Cadm1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

chicken beta-actin

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only
pCIG-VP16–RAR-alpha

Plasmid

#80434

Purpose

Expression of constitutively active human RAR-alpha in chick embryos

Depositor

Aixa Morales

Article

Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

Insert

Retinoic acid receptor alpha (RARA Human)

Use

Tags

EGFP and VP16

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only
pBa.GFP-KIF1Balpha tail 387-1150

Plasmid

#134619

Purpose

Mammalian expression of mouse KIF1Balpha 387-1150

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1Balpha (Kif1b Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 386 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Venus-MDGA1

Plasmid

#82543

Purpose

Overexpression vector for Venus-MDGA1 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

MDGA1 (MDGA1 Human)

Use

Tags

Venus fluorescent protein

Expression

Mammalian

Mutation

Promoter

chicken beta actin

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCIG-V5-Snail2-IRES-nls-GFP

Plasmid

#44282

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

V5-Snail2 (SNAI2 Chicken)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV-IE-enhancer and chicken beta-actin promoter

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-Venus-MDGA2

Plasmid

#82544

Purpose

Overexpression vector for Venus-MDGA2 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

MDGA2 (Mdga2 Rat)

Use

Tags

Venus fluorescent protein

Expression

Mammalian

Mutation

Promoter

chicken beta actin

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBa-FRB-3myc-KIF13A tail 361-1749

Plasmid

#64288

Purpose

KIF13A aa 361-1749 with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

KIF13A (Kif13a Mouse)

Use

Tags

3myc and FRB

Expression

Mammalian

Mutation

aa361-1749

Promoter

chicken Beta Actin

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBa-FRB-3myc-KIF13B tail 442-1826

Plasmid

#64289

Purpose

KIF13A aa 442-1826 with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

KIF13B (Kif13b Mouse)

Use

Tags

3myc and FRB

Expression

Mammalian

Mutation

aa 442-1826

Promoter

chicken Beta Actin

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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pIS1-Vim/Actb5UTR-renilla

Plasmid

#38238

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Vim promoter with Actb 5UTR/Renilla (Vim, Actb Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Actb promoter (1000 nt upstream) and 5' UTR …

Promoter

Endogenous Vim

Available since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
pBa.FRB-3myc-GS-KIF3A tail 419-702

Plasmid

#134614

Purpose

Mammalian expression of human KIF3A 419-702

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF3A (KIF3A Human)

Use

Tags

FRB, 3myc

Expression

Mammalian

Mutation

WT with deletion of the 418 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCIG-Flag-Sox9-IRES-nls-GFP

Plasmid

#44281

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

Flag-Sox9 (SOX9 Chicken)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV-IE enhancer and Chicken beta-actin promoter

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only