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Plasmid#112924PurposepiggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant markerDepositorInsertEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
UsePiggybac transposon vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-C-CreintG
Plasmid#69571PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1Insertkozak consensus - C-CreintG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-Puro
Plasmid#115143PurposeSafe harbor site 231 knock-in vector with puromycin resistance selection cassetteDepositorInsertPuromycin
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-Usp9x_CD-mCherry
Plasmid#170380PurposeVector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.DepositorInsertUbiquitin specific protease 9x catalytic domain (Usp9x Mouse)
UseTagsmCherry-3xFlagExpressionMammalianMutationCatalytic domainPromoterAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-Backbone(NG)
Plasmid#27963DepositorInsertTAL-Backbone(NG)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEF1alpha-mClover3-bGHpA
Plasmid#172286PurposeConstitutive expression of mClover3DepositorInsertmClover3
UseTagsExpressionBacterialMutationPromoterEF1alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PlayBack-Ef1a-EcoR1
Plasmid#203311PurposeFor expression of eukaryotic genes of interest with Ef1a promoter with EcoRI as a PlayBack Restriction EnzymeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-Cas9GFP-W
Plasmid#200100PurposeLentiviral vector expressing Cas9 fused with GFP at the C-terminusDepositorInsertGFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-LIC
Plasmid#120247PurposeFor LIC cloning with N-terminal V5 tagDepositorTypeEmpty backboneUseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHL-EF1a-SphcCas9-iP-A
Plasmid#60599PurposeExpresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.DepositorInsertCRISPR Cas9
UseCRISPRTagsExpressionMammalianMutationCodon usage optimized for human usagePromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHeEf1a-mEGFP-NLS
Plasmid#205012PurposeThe vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#112922PurposeGateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant markerDepositorInsertEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF1alpha-rtTA-Antares
Plasmid#197587PurposeExpresses TET-On reverse transactivator labeled with AntaresDepositorInsertrtTA-P2A-Antares
UseTagsAntaresExpressionMammalianMutationPromoterEF-1alphaAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_FL MUT TET1
Plasmid#124081PurposeExpression of catalytic inactive form of full-length TET1DepositorInsertTET1 - full length, mutated (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationH1672Y, D1674APromoterEF1 alphaAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-N-cG-Neo
Plasmid#124978PurposeExpress Nanoluc, copGFPDepositorInsertNanoluc, copGFP
UseLentiviralTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-tdTomato
Plasmid#122101PurposeAAV-mediated expression of tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.DepositorInserttdTomato
UseAAVTagsNAExpressionMammalianMutationPromoterEF1a(1.1kb short version)Available sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1alphaL-hAAT-W
Plasmid#24527PurposeLentiviral overexpressionDepositorInsertalpha-1 antitrypsin (SERPINA1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHR_EF1a-->sGFP_PGK-->puroR
Plasmid#162156PurposeLentiviral vector for constitutive expression of GFP in mammalian cellsDepositorInsertEGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
R777-E223 Hs.RAPGEF1
Plasmid#70507PurposeGateway ORF clone of human RAPGEF1 [NM_198679.1] with stop codon (for native or N-terminal fusions)DepositorInsertRAPGEF1 (RAPGEF1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLacO-pEF1α-RFP
Plasmid#179507PurposeExpresses monomeric RFP in mammalian cells under a EF1α promoterDepositorInsertmonomeric RFP
UseTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only