We narrowed to 9,299 results for: yeast expression

1. pCM184-ParB2::eGFP

   Plasmid

   #87252

   Purpose

   Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   ParB2
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   pBAD/tetO

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCM189-ParB1::mCherry

   Plasmid

   #87253

   Purpose

   Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   ParB1
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   pBAD/tetO

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOsTIR1w/oGFP

   Plasmid

   #102883

   Purpose

   Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   OsTIR1
   

   Use

   Tags

   3x myc

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRCC-K

   Plasmid

   #81191

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPRyl

   Plasmid

   #70007

   Purpose

   CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2015 Dec 29.

   Inserts

   Codon optimized Cas9 from S. pyogenes
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. TurboID-V5_pRS415

   Plasmid

   #107167

   Purpose

   expresses V5-tagged TurboID in the yeast cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRCC-N

   Plasmid

   #81192

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIW601-KmCRISPR

   Plasmid

   #98907

   Purpose

   K. marxianus CRISPR Plasmid for sgRNA cloning

   Depositor

   Ian Wheeldon

   Article

   Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

   Inserts

   Codon optimized Cas9
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCTCON2 Aga2P-HRP

   Plasmid

   #73152

   Purpose

   Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   Aga2P-HRP
   

   Use

   Tags

   HA tag and myc tag

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPEY-GAL-Nat

    Plasmid

    #232097

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. p415-PtrpC-Cas9-TtrpC-CYC1t

    Plasmid

    #68059

    Purpose

    Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    humanized Cas9
    

    Use

    CRISPR; N. crassa, fungi

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSc-humanADAR2

    Plasmid

    #171106

    Purpose

    Expresses human ADAR2 in yeast.

    Depositor

    Brenda Bass

    Article

    Macbeth et al Methods Enzymol. 2007;424:319-31. doi: 1

    Insert

    human ADAR2 (ADARB1 Human)
    

    Use

    Tags

    His12_TEV

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPEY-GAL-Kan

    Plasmid

    #232099

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPEY-GAL-Hyg

    Plasmid

    #232098

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPEY-Z3-Nat

    Plasmid

    #232100

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDS220

    Plasmid

    #34980

    Purpose

    Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb–mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7-EGFP-C1-HsNXF-1_K

    Plasmid

    #146740

    Purpose

    Mammalian Expression of HsNxf-1

    Depositor

    Elisa Izaurralde

    Article

    Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

    Insert

    HsNxf-1 (NXF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S144G relative to NM_006362.4

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIW447-CRISPR XYL2

    Plasmid

    #98906

    Purpose

    K. marxianus CRISPR Plasmid for XYL2 editing

    Depositor

    Ian Wheeldon

    Article

    Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

    Inserts

    Codon optimized Cas9
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS406 ATG9 stopGFP

    Plasmid

    #114669

    Purpose

    Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

    Insert

    ATG9
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Changed the three linker base pairs after the las…

    Promoter

    ATG9

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS406-PHO5-GFP-OSBP PH domain

    Plasmid

    #58840

    Purpose

    Expresses GFP-Tagged OSBP PH domain in yeast

    Depositor

    Tim Levine, Sean Munro

    Article

    Levine et al Curr Biol. 1998 Jun 18;8(13):729-39.

    Insert

    Oxysterol-binding Protein PH domain (OSBP Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    PHO5

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only