We narrowed to 166,761 results for: Gene

1. AAV8R-12 Capsid

   Plasmid

   #213968

   Purpose

   AAV packaging vector carrying AAV2 rep gene and Cap8R modified AAV8 capsid coding sequence

   Depositor

   Zhonghua Lu

   Article

   Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

   Type

   Empty backbone

   Use

   AAV

   Available Since

   March 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. kiCAP-AAV-BI-hTFR1

   Plasmid

   #218796

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al Science. 2024 Jun 14;384(6701):1220-1227

   Insert

   AAV Rep-Cap BI-hTFR1
   

   Expression

   Mammalian

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPR-v2-HygR-EGFP

   Plasmid

   #167188

   Purpose

   Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

   Depositor

   Yin Shen

   Article

   Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

   Insert

   Hygromycin B phosphotransferase and EGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHEE401E

   Plasmid

   #71287

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   CRISPR; Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-TetO-hNGN2-Neo

   Plasmid

   #99378

   Purpose

   Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   hNGN2-P2A-Neo (NEUROG2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TetO

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. TriEx-4 DHX36

   Plasmid

   #68368

   Purpose

   Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.

   Depositor

   James Vaughn

   Article

   Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2

   Insert

   DHX36 (DHX36 Human)
   

   Tags

   Histidine

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV ie enhancer/promoter

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

7. lentiCRISPR-v2-HygR-mCherry

   Plasmid

   #167189

   Purpose

   Lentiviral expression of sgRNA with hygromycin resistance gene and mCherry

   Depositor

   Yin Shen

   Article

   Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

   Insert

   Hygromycin B phosphotransferase and mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRM166 LKB (Luciferase mKate2 Blast)

   Plasmid

   #183502

   Purpose

   Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   Firefly Luciferase
   mKate2
   BSD
   

   Use

   Lentiviral and Luciferase

   Tags

   P2A linker and SV40 NLS with GGS linker

   Expression

   Mammalian

   Promoter

   MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC-G418-YR

   Plasmid

   #61767

   Purpose

   Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).

   Depositor

   Ken Haynes

   Article

   Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

   Inserts

   nptII gene
   2 micron origin or replication and URA3 gene for S. cerevisiae
   

   Use

   Ternary vector for agrobacterium mediated transfo…

   Promoter

   trpC promoter

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. p5xQUAS-ZsGreen-P2A, α-cry:mCherry

    Plasmid

    #180477

    Purpose

    Inducible gene expression vector p5xQUAS-ZsGreen-P2A, α-cry:mCherry

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Inserts

    5x QUAS-ZsGreen-P2A
    mCherry
    

    Use

    Synthetic Biology

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

    Plasmid

    #111818

    Purpose

    Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    Mef2c (Mef2c Mouse)
    Tdtomato
    Gata4 (Gata4 Mouse)
    Tbx5
    

    Use

    Cloning intermediate

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHEE401

    Plasmid

    #71286

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    sgRNA scaffold
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. p13xQUAS-ZsGreen-P2A, α-cry:mCherry

    Plasmid

    #180480

    Purpose

    Inducible gene expression vector p13xQUAS-ZsGreen-P2A, α-cry:mCherry

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Insert

    13x QUAS-ZsGreen-P2A
    

    Use

    Synthetic Biology

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. p13xQUAS-MCS, α-cry:mCherry

    Plasmid

    #180481

    Purpose

    Inducible gene expression vector p13xQUAS-MCS, α-cry:mCherry

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Insert

    Multiple cloning site
    

    Use

    Synthetic Biology

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-SV40NLSf-mScarletP2A-hLuc

    Plasmid

    #218793

    Purpose

    AAV reporter genome plasmid

    Depositor

    Ben Deverman

    Article

    Huang et al Science. 2024 Jun 14;384(6701):1220-1227

    Insert

    NLSf-mScarletP2A-hLuc
    

    Use

    AAV

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQFDBD-2x AD*-VP16*, α-cry:EGFP

    Plasmid

    #180473

    Purpose

    Gene expression vector pQFDBD-2x AD*-VP16*, α-cry:EGFP

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Insert

    QFDBD-2xQFAD*-VP16*
    

    Use

    Synthetic Biology

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. p9xQUAS-ZsGreen-P2A, α-cry:mCherry

    Plasmid

    #180479

    Purpose

    Inducible gene expression vector p9xQUAS-ZsGreen-P2A, α-cry:mCherry

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Insert

    9x QUAS-ZsGreen-P2A
    

    Use

    Synthetic Biology

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. p17xQUAS-Luciferase, α-cry:mCherry

    Plasmid

    #180482

    Purpose

    Inducible gene expression vector p17xQUAS-Luciferase, α-cry:mCherry

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Inserts

    Luciferase
    17x QUAS
    

    Use

    Synthetic Biology

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

    Plasmid

    #64222

    Purpose

    Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiV_Neo_POU2F3_CR

    Plasmid

    #122175

    Purpose

    Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

    Insert

    POU2F3 (POU2F3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    3 silent mutations for gRNA resistance (882g-c, 8…

    Promoter

    EFS

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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