We narrowed to 13,844 results for: cas9 genes

1. PX330-sgRNA006_CTCF

   Plasmid

   #232928

   Purpose

   Expression vector for a sgRNA against the mouse CTCF locus and SpCas9.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   Cas9 (Ctcf S. pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPRISM-QF2-he1.1:YFP

   Plasmid

   #184819

   Purpose

   Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.

   Depositor

   Marnie Halpern

   Article

   Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

   Insert

   QF2-pA-he1.1:YFP
   

   Use

   CRISPR; Backbone for cloning of homology arms (fo…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMpGE010-Target1 (Mpphot)

   Plasmid

   #186728

   Purpose

   Binary vector for CRISPR/Cas9 (target 1: Mpphot [positive control]) in plants (for Agrobacterium-mediated genetic transformation).

   Depositor

   Yutaka Kodama

   Article

   Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

   Insert

   Mphot
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMpGE010-Target2 (Mpphot)

   Plasmid

   #186729

   Purpose

   Binary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation).

   Depositor

   Yutaka Kodama

   Article

   Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

   Insert

   Mphot
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMpGE010-sgRNA-Target1 (MpRTN1)

   Plasmid

   #186295

   Purpose

   Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

   Insert

   sgRNA-Target1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDGE148

   Plasmid

   #79450

   Purpose

   recipient plasmid [multiplexing]; pDD45:Cas9, FAST, BASTA

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   ccdB (ccdB(letD) )
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDGE151

   Plasmid

   #79451

   Purpose

   recipient plasmid [multiplexing]; pICU2:Cas9, FAST, BASTA

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   ccdB (ccdB(letD) )
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDGE164

   Plasmid

   #79454

   Purpose

   recipient plasmid [multiplexing]; pDD45:Cas9, FAST, nptII

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   ccdB (ccdB(letD) )
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDGE165

   Plasmid

   #79455

   Purpose

   recipient plasmid [multiplexing]; pICU2:Cas9, FAST, nptII

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   ccdB (ccdB(letD) )
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDGE169

    Plasmid

    #79459

    Purpose

    recipient plasmid [multiplexing]; pICU2:Cas9, FAST, Hygro

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB (ccdB(letD) )
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK384.4

    Plasmid

    #155369

    Purpose

    dCas9 + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK412

    Plasmid

    #155371

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRDA_336

    Plasmid

    #179095

    Purpose

    BE Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    BE3.9 (Cas9-NG)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAID-EF1a linearizing in pX330

    Plasmid

    #140610

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-EF1a

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEF-AncBE4max

    Plasmid

    #138270

    Purpose

    Expression of AncBE4max-Cas9 Base-editor from an EF1a promoter.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    AncBE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Ef1a

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRDA_429

    Plasmid

    #179098

    Purpose

    BE Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    ABE8e (Cas9-NG)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. px458_2A_GFP_sgRNA_TIA1

    Plasmid

    #106097

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIA1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA TIA1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lv EF1a MS2-Fkbp2x 2A Hygro

    Plasmid

    #102807

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp2x fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    MS2-Fkbp2x
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lv EF1a SS18-Frb PGK Puro

    Plasmid

    #102811

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express SS18-Frb fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    SS18-Frb
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. px458_2A_GFP_sgRNA_TIAL1

    Plasmid

    #106096

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIAL1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA TIAL1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUCmini-UGCG-tdTomato

    Plasmid

    #225879

    Purpose

    Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertion

    Depositor

    Yohei Sato

    Article

    Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

    Insert

    tdTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. piCRg Entry

    Plasmid

    #58904

    Purpose

    Cas9/gRNA Entry expression plasmid

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    hSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pADH100

    Plasmid

    #90980

    Purpose

    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMJ823

    Plasmid

    #39313

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9 (lin2744 Listeria innocua)
    

    Use

    CRISPR

    Tags

    MBP and thioredoxin-His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pADH118

    Plasmid

    #90983

    Purpose

    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. albicans LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lv EF1a HP1cs-Frb1x PGK Puro

    Plasmid

    #102808

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb1x fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    HP1cs-Frb1x
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pADH143

    Plasmid

    #90989

    Purpose

    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. mal LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LT0

    Plasmid

    #210551

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT0::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LT1

    Plasmid

    #210552

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT1::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LT2

    Plasmid

    #210553

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT2::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMJ839

    Plasmid

    #39317

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9 (NMA0631 Neisseria meningitidis)
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

32. Lv EF1a HP1cs-Frb2x PGK Puro

    Plasmid

    #102809

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb2x fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    HP1cs-Frb2x
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Lv EF1a MS2-Fkbp1x 2A Hygro

    Plasmid

    #102806

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp1x fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    MS2-Fkbp
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pORE303N_CEN12RRFuzzy3MYB

    Plasmid

    #194441

    Purpose

    CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYB

    Depositor

    Chung-Jui Tsai

    Article

    Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

    Insert

    gRNA array targeting CEN1, RR, Fuzzy3MYB
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    MtU6

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRDA_275

    Plasmid

    #179091

    Purpose

    nuclease Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    Cas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEF-BE4Gam

    Plasmid

    #138269

    Purpose

    Expression of BE4am-Cas9 Base-editor from an EF1a promoter.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    BE4Gam
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Ef1a

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAID-CMV linearizing in pX330

    Plasmid

    #140609

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-CMV

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pX330(puro) Rosa26-H3F3B

    Plasmid

    #73131

    Purpose

    Expression in Mammalian Cells, Cas9 Nuclease to analize translocations

    Depositor

    Agnel Sfeir

    Article

    Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

    Insert

    spCas9
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Lv EF1a MS2-HP1cs 2A Hygro

    Plasmid

    #102810

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express MS2-HP1cs fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    MS2-HP1cs
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRDA_156

    Plasmid

    #179089

    Purpose

    nuclease Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    Cas9-VRER
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge