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UbG76V-eGFP-V5His_15aa

Plasmid

#23969

Depositor

Nico Dantuma

Article

Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

Insert

Ub-G76V-eGFP-V5HIS_15aa (UBB Human)

Tags

eGFP

Expression

Mammalian

Mutation

G76V mutation in Ubiquitin. Ub fused to N-termin…

Available Since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
p6347 MSCV-CMV-Flag-HA-Brd4-444-722

Plasmid

#31353

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 444-722 (BRD4 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pCaSpeR4 mito roGFP2-Grx1

Plasmid

#65000

Purpose

Expression of mitochondrial roGFP2-Grx1 under the alphaTub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pUAST mito roGFP2-Grx1

Plasmid

#64995

Purpose

for expression of mitochondrial roGFP2-Grx1 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-siResist

Plasmid

#49852

Purpose

Encodes human connexin 43 with wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Contains several wobble mutations to confer resis…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
pRetroX-Tight-Pur-Trim69 isoform B (codon optimized)

Plasmid

#199567

Purpose

Stable and dox. inducible expression of Trim69 isoform B (codon optimized) after retroviral-mediated gene transfer

Depositor

Andrea Cimarelli, Yuxin Song

Article

Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

Trim69 isoform B (codon optimized) (TRIM69 Human)

Use

Retroviral; Allows for puromycin selection

Tags

Flag

Mutation

codon optimized

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-AcGFP-P2A-hBRD4S

Plasmid

#197882

Purpose

study roles of human BET proteins in human pluripotency reprogramming

Depositor

Kejin Hu

Article

Hossain et al iScience. 2022 Dec 28;26(1):105889. doi:

Insert

BRD4 (BRD4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsDCP1a_G

Plasmid

#146400

Purpose

Mammalian Expression of HsDcp1a

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDcp1a (DCP1A Human)

Expression

Mammalian

Mutation

two silent mutations T237C and G1716T compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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p6349 MSCV-CMV-Flag-HA-Brd4 1224-1362

Plasmid

#31355

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 1224-1362 (BRD4 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-DNAJC5 (L115R)

Plasmid

#205728

Purpose

Mammalian expression of DNAJC5 (L115R)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (L115R) (DNAJC5 Human)

Use

Lentiviral

Mutation

L115R

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term

Plasmid

#200634

Purpose

Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing pad

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

PTEN (PTEN Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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TOPO UBC

Plasmid

#68456

Purpose

TOPO construct expressing UBC for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Ubiquitin C Promoter (UBC Human)

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Pb-TRE3G-TYR-HygR

Plasmid

#195508

Purpose

inducible Piggybac vector expressing human TYR CDS with hygromycin selection

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

TYR (TYR Human)

Use

Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP5.v2 puro

Plasmid

#87793

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP5.v1 puro

Plasmid

#87792

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-TUBA1B_sgRNA

Plasmid

#183888

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

TUBA1B sgRNA spacer (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Bmi1

Plasmid

#61532

Purpose

Expresses Bmi1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Bmi1 (Bmi1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
p260 pCMV-CRE-M-AR(wt)

Plasmid

#12494

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

Insert

CRE-M AR (AR Human)

Use

Cre/Lox

Expression

Mammalian

Mutation

CRE-M and AR ligand binding domain

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pet151D-TOPO BROX 1-411;

Plasmid

#89602

Purpose

Expresses full length human Brox in bacteria; internal ID WISP11-297

Depositor

Wesley Sundquist

Article

Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

Insert

BROX (BROX Human)

Tags

His Tag, V5 tag

Expression

Bacterial

Promoter

T7

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-GFP

Plasmid

#82506

Purpose

Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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