We narrowed to 44,511 results for: REP

1. pFUW-tetO-GATA2

   Plasmid

   #125028

   Purpose

   doxycycline-inducible expression of human GATA2 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

   Insert

   GATA binding protein 2 (GATA2 Human)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-mCMV

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGAMA-YAP

   Plasmid

   #74942

   Purpose

   Lentiviral over-expression vector for YAP

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   T2A-mCherry

   Expression

   Mutation

   Promoter

   EF1α

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMy-MouseCTCFbiotag-T2A-mOrange

   Plasmid

   #50564

   Purpose

   Retroviral vector endoding for mOrange and mouse CTCF with a short biotinylation substrate (biotag, Kimetal.,2009) fused to its C-term

   Depositor

   Rafael Casellas

   Article

   Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

   Inserts

   Ampicilin
   Mouse CTCF Exons
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57-mCherry-2A-BRD4 Iso A

   Plasmid

   #137720

   Purpose

   Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform

   Depositor

   Scott Floyd

   Article

   Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

   Insert

   BRD4 long isoform (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL7 lenti-dcas9-3xflag-blast

   Plasmid

   #112133

   Purpose

   lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   dCas9(D10A, N863A)-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A mutants in Cas9

   Promoter

   EF1a

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70

   Plasmid

   #62953

   Purpose

   Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   3XGal80-FLAG/HA/EGFP-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE

   Plasmid

   #122056

   Purpose

   Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253P

   Depositor

   Stephen Tapscott

   Article

   Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

   Insert

   MD(ND2bHLH)WCS (Myod1, Neurod2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   MyoD chimeric protein substituted with the NeuroD…

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1179_pAAV-U6-BbsI-gRNA-CB-EmGFP

   Plasmid

   #89060

   Purpose

   AAV-gRNA cloning vector with GFP reporter

   Depositor

   William Lagor

   Article

   Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

   Insert

   EmGFP
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin with partial CMV enhancer

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. LAMP-mChF-AIP

   Plasmid

   #61524

   Purpose

   Expression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIP

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Insert

   lysosomal-associated membrane protein 1 (LAMP1 Human)
   

   Use

   Tags

   AIP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-Flag-MIB1-IRES-Puro

    Plasmid

    #140240

    Purpose

    Lentiviral vector expressing flag-tagged MIB1

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    Flag-MIB1 (MIB1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUW-tetO-GFI1B

    Plasmid

    #125597

    Purpose

    doxycycline-inducible expression of human GFI1B in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

    Insert

    Growth Factor Independent 1B transcriptional repressor (GFI1B Human)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. mChF-AIP

    Plasmid

    #61527

    Purpose

    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    FK506 binding protein 1A (FKBP1A Human)
    

    Use

    Tags

    AIP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pVITRO1-Trastuzumab-IgG3/κ

    Plasmid

    #61885

    Purpose

    Expresses HER2/neu receptor specific humanized IgG3/κ antibody isotype (Trastuzumab-IgG3/κ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    HER2/neu receptor specific humanized Gamma 3 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mEF1 Prom and rEF1 Prom

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57-mCherry-2A-BRD4 Iso C

    Plasmid

    #137721

    Purpose

    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 short isoform

    Depositor

    Scott Floyd

    Article

    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

    Insert

    BRD4 short isoform (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pScalps_Puro_mCebpa

    Plasmid

    #79551

    Purpose

    Expression of mouse Cebpa

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Cebp alpha (Cebpa Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA

    Plasmid

    #105000

    Purpose

    Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TetCMV

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-COSMC-KO

    Plasmid

    #80008

    Purpose

    gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

    Depositor

    Sriram Neelamegham

    Article

    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

    Insert

    COSMC (C1GALT1C1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICE-HA-Ku70-siR-WT

    Plasmid

    #82329

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10

    Insert

    Ku70 (XRCC6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tet

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST40-2XFL-Src-S4-S32-KD

    Plasmid

    #140299

    Purpose

    Expresses Flag-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    lacks the UD region

    Promoter

    CMV

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR221-IL6.Pro

    Plasmid

    #79491

    Purpose

    MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human IL6 promoter

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    Il6 Promoter (IL6 Human, Synthetic)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    IL6 Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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