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163,403 results

Showing: 8011 - 8040 of 163403 results

  3. mCherry-Peroxisomes-2

    Plasmid
    #54520
    Purpose
    Localization: Peroxisomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    Peroximal Targeting Signal 1 (PTS1) and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54520-plasmid-map-sequence-id-337369

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  7. pSpCas9(BB)-2A-Neo

    Plasmid
    #127762
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127762-plasmid-map-sequence-id-247554

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  12. CIDAR MoClo Extension, Volume I

    Plasmid Kit
    #1000000161
    Purpose
    This kit contains 110 plasmids designed to quickly and efficiently build a wide variety of gene expression constructs using the CIDAR-standard MoClo system.
    Depositor
    Richard Murray
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only

  13. pT7TS-rich

    Plasmid
    #99050
    Purpose
    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.
    Depositor
    Richard Bélanger
    Type
    Empty backbone
    Use
    Unspecified; Xenopus oocyte system
    Tags
    Expression
    Mutation
    Promoter
    pT7
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    99050-plasmid-map-sequence-id-191760

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  17. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid
    #140001
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140001-plasmid-map-sequence-id-477414

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  21. GPR37-DuET

    Plasmid
    #213282
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR37 (GPR37 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213282-plasmid-map-sequence-id-430904

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  24. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

    Plasmid
    #174081
    Purpose
    Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.
    Depositor
    Ryan Mehl
    Article
    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-
    Insert
    NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
    Use
    Tags
    NES-Flag
    Expression
    Mammalian
    Mutation
    L270G, Y271L, N311G, C313A
    Promoter
    CMV
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174081-plasmid-map-sequence-id-345715

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  26. pICE-RNaseHI-WT-NLS-mCherry

    Plasmid
    #60365
    Purpose
    Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Patrick Calsou, Sébastien Britton
    Article
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Insert
    RNase HI
    Use
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60365-plasmid-map-sequence-id-256737

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  27. pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (AAV1)

    Viral Prep
    #75470-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (#75470). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry plasmid DNA. CAG-driven, Flp recombinase-dependent expression of channelrhodopsin H134R mutant fused to mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Connie Cepko
    Article
    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:
    Promoter
    CAG
    Tags
    mCherry
    Available since
    March 2, 2020
    Availability
    Academic Institutions and Nonprofits only

  28. pGL4.23-MYC

    Plasmid
    #86461
    Purpose
    A modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site.
    Depositor
    Eric Lander
    Article
    Fulco et al Science. 2016 Sep 29. pii: aag2445.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MYC
    Available since
    Sept. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86461-plasmid-map-sequence-id-193100

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Showing: 8011 - 8040 of 163403 results