We narrowed to 10,638 results for: ESP

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sgPBRM1-2- LentiCRISPRv2

Plasmid

#107404

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero45

Plasmid

#187931

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero45

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero45 (SERBP1 Human)

Tags

Flag-6xHis

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-MAPKAPK2

Plasmid

#82720

Purpose

Expresses human MAPKAPK2

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MAPKAPK2 (MAPKAPK2 Human)

Tags

3xFLAG and GST

Expression

Bacterial

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pCMMP-dnPERK-IRES-eGFP

Plasmid

#36954

Depositor

Bill Sugden

Article

Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

Insert

dnPERK (EIF2AK3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

deletion of aa592-1061 to create dominant negative

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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His-SUMO-hDDI2_pET11a

Plasmid

#169120

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Expression

Bacterial

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_ALDH7A1

Plasmid

#106120

Purpose

Lentiviral expression of ALDH7A1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

ALDH7A1 (ALDH7A1 Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gT28.4.hSyn.H2B.RFP

Plasmid

#170387

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-hSTING (S366A)

Plasmid

#214163

Purpose

Retroviral transduction of STING

Depositor

Nan Yan

Article

Wu et al Immunity. 2020 Jul 14;53(1):115-126.e5.

Insert

STING (S366A) (STING1 Human)

Use

Retroviral

Mutation

S366A

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-hSTING (R232)

Plasmid

#214162

Purpose

Retroviral transduction of STING

Depositor

Nan Yan

Article

Wu et al Immunity. 2020 Jul 14;53(1):115-126.e5.

Insert

STING (STING1 Human)

Use

Retroviral

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET_MBP-RGG-GFP-RGG

Plasmid

#124949

Purpose

Expression of MBP-RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

MBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

EGFP, His, and MBP

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAT003

Plasmid

#171628

Purpose

2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-TRAC
mNeon

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and EF1-a

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-UBC-hAAT-IRES-ZsGreen-W

Plasmid

#126702

Purpose

lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen

Depositor

Darrell Kotton

Article

Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

Insert

Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)

Use

Lentiviral

Promoter

UBC

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-mPERK-FLAG-IP

Plasmid

#101793

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse PERK

Depositor

Seiichi Oyadomari

Article

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Perk (Eif2ak3 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1

Plasmid

#19838

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pLGB36

Plasmid

#135621

Purpose

Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection

Depositor

Laurie Comstock

Article

Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-

Insert

bfe3

Use

Bacteroides suicide vector with inducible counter…

Tags

signal sequence for periplasmic targeting

Promoter

Bacteroides promoter regualted by TetR transcript…

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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NAE1 G8.3 gRNA

Plasmid

#90777

Purpose

3rd generation lentiviral gRNA plasmid targeting human NAE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NAE1 (Guide Designation G8.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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p4XBRE-SBE-SV40-GL3

Plasmid

#67811

Purpose

luciferase reporter with mouse Id1 promoter fragments

Depositor

Ron Prywes

Article

Lewis et al Biochim Biophys Acta. 2013 Oct;1829(10):

Insert

Id1 (Id1 Mouse)

Tags

luciferase

Expression

Mammalian

Promoter

Id1/SV40

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only
VEGFR2-YFP

Plasmid

#108853

Purpose

Encodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker

Depositor

Kalina Hristova

Article

Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

Insert

KDR (KDR Human)

Tags

Labeled with eYFP on the C-Terminus via a 3 amino…

Expression

Mammalian

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_mSTING_scrambled_gRNA_2

Plasmid

#196628

Purpose

Scrambled control 2 for STING knock-out in murine cells.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING scrambled gRNA 2

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-SPORT6-RNF114-RINGmut

Plasmid

#69922

Purpose

Expresses mutant myc-RNF114 in mammalian cells

Depositor

Francesca Capon

Article

Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

Insert

RNF114 (RNF114 Human)

Expression

Mammalian

Mutation

C29A, C32A

Promoter

CMV

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only