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actc1b-mKate2-RhoAa-DN

Plasmid

#109526

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative RhoAa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAa (rhoaa Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_A2_G_31AACTdel

Plasmid

#237352

Purpose

Expression plasmid for RSV A2 G

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV A2 G human codon optimized from Genbank Accesion KT992094.1

Expression

Mammalian

Mutation

31 amino acid cytoplasmic tail deletion

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_B1_G_31AACTdel

Plasmid

#237354

Purpose

Expression plasmid for RSV B1 G

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV B1 G human codon optimized from Genbank Accesion AF013254

Expression

Mammalian

Mutation

31 amino acid cytoplasmic tail deletion

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

Plasmid

#162335

Purpose

Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-RhoAb-DN

Plasmid

#109580

Purpose

Multisite gateway vector for 3' tagging with dominant negative RhoAb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAb (rhoab Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgNFkB2 guide 1

Plasmid

#193593

Purpose

NFkB2 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgNFkB2 guide 1 (NFKB2 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SGSM3[1-723]

Plasmid

#246258

Purpose

Rab5 GAP

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

Sgsm3[1-723] (Sgsm3 Rat)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCdrA-gfp(ASV)C

Plasmid

#111615

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(ASV)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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GA-NES

Plasmid

#61018

Purpose

ddGFP A-NES

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-P2A-APEX2-VHH[GFP]

Plasmid

#126749

Purpose

Expression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

VHH[EGFP]

Use

Zebrafish muscle expression clone

Tags

mKate2-P2A-APEX2

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

Plasmid

#83919

Purpose

Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

DsRed-Express2
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Promoter

hUbC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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mVenusC-Cavin3

Plasmid

#108890

Purpose

Expression of Cavin3 fused to the C-terminus of mVenus for bimolecular fluorescence complementation

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

Cavin3 (altered codon usage) (CAVIN3 Synthetic, Human)

Tags

mVenus156-239

Expression

Mammalian

Mutation

altered codon usage

Available Since

March 23, 2019

Availability

Academic Institutions and Nonprofits only

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mVenusN-Cavin3

Plasmid

#108889

Purpose

Expression of Cavin3 fused to the N-terminus of mVenus for bimolecular fluorescence complementation

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

Cavin3 (altered codon usage) (CAVIN3 Synthetic, Human)

Tags

mVenus1-155

Expression

Mammalian

Mutation

altered codon usage

Available Since

March 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTn7CdrA-gfp(ASV)C

Plasmid

#111617

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(ASV)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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actc1b-2PH-PLCd-mKate2

Plasmid

#109500

Purpose

Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-2PH-PLCd-mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPHT-PIP2

Plasmid

#60937

Purpose

Fluorescent FPX sensor for PIP2

Depositor

Anne Marie Quinn

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

PIP2 dimerization dependent sensor RA+B monomers

Tags

dimerization dependent fluorescent protein monome…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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RA-NES

Plasmid

#61019

Purpose

ddRFP A-NES

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddRFP A

Tags

HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pTn7CdrA-gfpC

Plasmid

#111616

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(Mut3)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pDN34-BiNLS-hYAP1-1delta

Plasmid

#169471

Purpose

expresses optoYAP in mammalian cells

Depositor

Timothy Saunders

Article

Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

Insert

YAP1-1delta (YAP1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

Plasmid

#162333

Purpose

Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

dSpCas9-2xVP64

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pTet-O-RUNX3-T2A-PuroR

Plasmid

#162349

Purpose

Lentiviral expression of RUNX3 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

RUNX3 (RUNX3 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG metap2

Plasmid

#32004

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

metap2 (Metap2 )

Tags

FLAG

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pME-loxP-mTagBFP2-stop-pA-loxP

Plasmid

#75158

Purpose

Floxed TagBFP2 (blue) fluorophore with stop codon for inducible lines.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

loxP-mTagBFP2-stop-pA-loxP

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTol2p2A--3mnx1:mTagBFP-NS-MCS

Plasmid

#78250

Purpose

Can be used to subclone in gene of interest into blue motor neuron expression vector.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Inserts

-3mnx1 (mnx1 Zebrafish)
-mTagBFP-NS-MCS

Use

Zebrafish motor neuron expression

Promoter

-3mnx1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

Plasmid

#83925

Purpose

Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

eGFP
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Promoter

hUbC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#162334

Purpose

Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

dSaCas9-KRAB

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hUbC

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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acta1-Cavin1a-Clover

Plasmid

#163852

Purpose

Zebrafish muscle specific expression of Cavin1a-Clover, Parton lab clone DPR

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Cavin1a (cavin1a Zebrafish)

Use

Zebrafish expression

Tags

Clover

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-Rac1-DN

Plasmid

#109582

Purpose

Multisite gateway vector for 3' tagging with dominant negative Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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ML1Nmut_in_pEGFP-C1

Plasmid

#67796

Purpose

Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2(7Q)-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-MLC2(CA)-IRES-GFP

Plasmid

#133928

Purpose

Bicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

EGFP and MLC2(CA) (MYL2 Human)

Expression

Mammalian

Mutation

MLC2 T18D, S19D

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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