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We narrowed to 8,720 results for: ef1

Showing: 521 - 540 of 8720 results

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pHAGE-EF1aL-nlsGFP-W

Plasmid

#126688

Purpose

Constitutive, ubiquitous, nuclear-localized GFP expression.

Depositor

Darrell Kotton

Article

Tang et al Nat. Phys. 18, 1371–1378 (2022).

Insert

nlsGFP

Use

Lentiviral

Tags

6xHIS and NLS

Expression

Mutation

Promoter

EF1aL

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a-EGFP-SKL

Plasmid

#170166

Purpose

Mammalian Expression of Ef1a-EGFP-SKL

Depositor

Christopher Moxham

Insert

EGFP-SKL

Use

Tags

Expression

Mammalian

Mutation

SKL

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1a-BFPCDK6-W

Plasmid

#208756

Purpose

Lentiviral vector expressing BFP fused with CDK6 cDNA

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CDK6 (CDK6 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-N-cG-Blast

Plasmid

#124981

Purpose

Express Nanoluc, copGFP

Depositor

Kazuhiro Oka

Insert

Nanoluc, copGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1-alpha

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-EF16

Plasmid

#52642

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF16

Use

Tags

Expression

Mammalian

Mutation

D431N/D433N/D435N/E442A mutated from GCaMP3

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-EF19

Plasmid

#52644

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF19

Use

Tags

Expression

Mammalian

Mutation

T328D/T330D mutated from GCaMP3

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
pHR_EF1a-->myc-mtC11L34

Plasmid

#162226

Purpose

Lentiviral vector for the expression of Myc mtC11L34 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-PNE scFv C11L34

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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mc2 mCherry EF1a

Plasmid

#206225

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-GFP

Plasmid

#122100

Purpose

AAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

GFP

Use

AAV

Tags

NA

Expression

Mammalian

Mutation

Promoter

EF1a（1.1kb short version)

Available since

May 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-EF1-GFP

Plasmid

#133018

Purpose

(Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under EF1 promoter.

Depositor

Michael Huen

Article

Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

Type

Empty backbone

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBS505 EF1alpha-EGFPcre*

Plasmid

#11955

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

EGFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
LV_EF1a_CDK4-P2A-Hygro_Barcode

Plasmid

#170223

Purpose

Barcoded lentiviral vector to express CDK4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-PM mir shLEF1 a

Plasmid

#27019

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir LEF1 3a

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-CCreV

Plasmid

#140132

Purpose

can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

CCreV

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NCreV

Plasmid

#140131

Purpose

can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

NCreV

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SOX2

Plasmid

#35388

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE-Sox2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
pRosa26-EF1a-hCas9IRESneo

Plasmid

#67987

Purpose

Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-hCas9IRESneopA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Backbone(NN)

Plasmid

#27961

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-Backbone(NN)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a/b-globin

Plasmid

#82582

Purpose

5' entry vector containing frog translation elongation factor 1 alpha (EF1a) enhancer fused to rabbit b-globin intron for semi-ubiquitous expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

Frog EF1a enhancer fused to rabbit b-globin intron

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pEF1a-2xSV40_NLS-NLuc

Plasmid

#135953

Purpose

Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Use

Tags

2xSV40-NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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