Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

167,318 results

Showing: 8131 - 8160 of 167318 results

  1. pPolI_SARS-CoV-2_NLuc

    Plasmid
    #183953
    Purpose
    Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter
    Depositor
    AJ te Velthuis
    Article
    Vial et al bioRxiv 2021.06.28.450211
    Insert
    SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
    Expression
    Mammalian
    Promoter
    Mammalian Pol I
    Available Since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183953-plasmid-map-sequence-id-365335

    Enlarge

  3. EBA140-bio

    Plasmid
    #47742
    Purpose
    Expresses enzymatically monobiotinylated full-length EBA140 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised EBA140
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47742-plasmid-map-sequence-id-68750

    Enlarge

  4. pSB167 - pL2_pSB90_2x35S::fLUC-I::tNOS_tMAS::rLUC-I::pMAS

    Plasmid
    #123200
    Purpose
    binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron) and a firefly luciferase (fLUC, with intron) in plants
    Depositor
    Carolyn Lee-Parsons, Erin Cram
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    2x35S::fLUC-I::tNOS tMAS::rLUC-I::pMAS
    Use
    Luciferase
    Expression
    Plant
    Available Since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123200-plasmid-map-sequence-id-242251

    Enlarge

  7. pHS2002

    Plasmid
    #239832
    Purpose
    Engineered OrufIscB (NovaIscB) omegaRNA mammalian expression
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    NovaIscB omegaRNA scaffold
    Expression
    Mammalian
    Mutation
    Replacement of A12-A57 with GAAA in omegaRNA scaf…
    Promoter
    human U6
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239832-plasmid-map-sequence-id-479110

    Enlarge

  8. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid
    #227274
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227274-plasmid-map-sequence-id-452035

    Enlarge

  14. pLentiCMV_HyPer7RgDAAO

    Plasmid
    #217653
    Purpose
    Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane
    Depositor
    Seth Parker
    Article
    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi
    Insert
    HyPer7 D amino acid oxidase
    Use
    Lentiviral
    Tags
    Nuclear export signal
    Expression
    Mammalian
    Mutation
    Fused DAAO to the C-terminus of HyPer7 using a Gl…
    Promoter
    CMV
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217653-plasmid-map-sequence-id-441459

    Enlarge

  21. EBA175-bio

    Plasmid
    #47743
    Purpose
    Expresses enzymatically monobiotinylated full-length EBA175 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised EBA175
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47743-plasmid-map-sequence-id-68751

    Enlarge

  23. pHBintE

    Plasmid
    #48136
    Purpose
    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector
    Depositor
    Dieter Jahn
    Article
    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
    48136-plasmid-map-sequence-id-69942

    Enlarge

  27. pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (AAV1)

    Viral Prep
    #100851-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (#100851). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 8131 - 8160 of 167318 results