We narrowed to 6,120 results for: nls
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Plasmid#221923PurposeExpression of CBP CFID fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP CFID (aa 1892-2441) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the CBP-FUS interaction domain (CFID…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR3-mCh-Cry2WT-NLS
Plasmid#221919PurposeExpression of CBP IDR3 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR3 (aa 441-587) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR3 region (aa 441 - 587); inse…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR7-mCh-Cry2WT-NLS
Plasmid#221922PurposeExpression of CBP IDR7 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR7 (aa 2109-2434) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR7 region (aa 2109 - 2434); in…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR6-mCh-Cry2WT-NLS
Plasmid#221921PurposeExpression of CBP IDR6 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR6 (aa 1856-2090) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR6 region (aa 1856-2090); inse…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR1-mCh-Cry2WT-NLS
Plasmid#221918PurposeExpression of CBP IDR1 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR1 (aa 72-175) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR1 region (aa 72 - 175); inser…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1P137 - pAHP6-PhiC31-NLS-tUBQ1
Plasmid#219702PurposeLevel 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integraseDepositorInsertpAHP6:PhiC31-NLS:tUBQ1
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:NLS-mApple-he1.1:CFP
Plasmid#184814PurposeUsed to generate the Tg(QUAS:NLS-mApple; he1.1:CFP)c718 transgenic lineDepositorInsertQUAS:NLS-mApple-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsNLS-mAppleExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:NLS-GFP-he1.1:CFP
Plasmid#184815PurposeUsed to generate the Tg(QUAS:NLS-GFP; he1.1:CFP)c682 transgenic lineDepositorInsertQUAS:NLS-GFP-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsNLS-GFPExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(48)-NLS-VSV
Plasmid#212746PurposeDoxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(63)-NLS-VSV
Plasmid#212756PurposeDoxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(63)-NLS-VSV
Plasmid#212755PurposeConstitutive expression of NUbo-E3(63)-NLS-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(48)-NLS-VSV
Plasmid#212745PurposeConstitutive expression of NUbo-E3(48)-NLS-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
A3Bctd-L7G-Cas9n-UGI-NLS
Plasmid#198886PurposeExpress A3Bctd-L7G BE3-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
UseTagsNLSExpressionMammalianMutationLoop 7 of A3GPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
A3Bctd-D314E-Cas9n-UGI-NLS
Plasmid#198887PurposeExpress A3Bctd-D314E BE3-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
UseTagsNLSExpressionMammalianMutationD314EPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NLS-MBP-rATOM(WDR5)
Plasmid#209710PurposeMammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signalDepositorInsertNLS-MBP-rATOM(WDR5)
UseTagsNLS-MBPExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HypaR-SpCas9-NLS-6xHis
Plasmid#207388PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HypaR-SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR661A, N692A, M694A, Q695A, H698A, amino acids 10…PromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-HypaR-SpCas9-NLS-6xHis
Plasmid#207387PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHypaR-SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR661A, N692A, M694A, Q695A, H698APromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-SpCas9-HF1-NLS-6xHis
Plasmid#207382PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags6xHisExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HiFi SpCas9-NLS-6xHis
Plasmid#207378PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HiFi SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR691A, amino acids 1005-1013 replaced with two gl…PromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-Blackjack SpCas9-NLS-6xHis
Plasmid#207375PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertBlackjack SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationamino acids 1005-1013 replaced with two glycinePromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆sap-NLS-3XFLAG-V5
Plasmid#196091PurposeExpresses mouse SAFB lacking the n-terminal SAP domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 31-65 (SAP domain) of mouse S…PromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆dd1-NLS-3XFLAG-V5
Plasmid#196092PurposeExpresses mouse SAFB lacking the first computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 96-285 (predicted disordered …PromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆rrm-NLS-3XFLAG-V5
Plasmid#196093PurposeExpresses mouse SAFB lacking the rrm domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 428-506 (RRM) of mouse SAFB p…PromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆dd3-NLS-3XFLAG-V5
Plasmid#196094PurposeExpresses mouse SAFB lacking the third computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 638-925 (predicted disordered…PromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-SAFB-DD3-NLS-3XFLAG-V5
Plasmid#196095PurposeExpresses only the third computationally called disordered domain of mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationContains only AA 619-925 (NLS and predicted disor…PromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoG-NAD-NLS3x
Plasmid#193822PurposeCMV overexpression of ChemoG-NAD in the nucleus of mammalian cell linesDepositorInsertChemoG-NAD-NLS3x
UseTagsExpressionMammalianMutationEGFP:A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO-ChemoL-NAD-NLS3x
Plasmid#193828PurposeCMV overexpression of ChemoL-NAD in the nucleus of mammalian cell linesDepositorInsertChemoL-NAD-NLS3x
UseTagsExpressionMammalianMutationEGFP:A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-alphaCaMKII-dsRed-Express-3XNLS-pA
Plasmid#183802PurposeAAV vector to express nuclear localized dsRed-Express from CaMKIIa promoterDepositorInsertdsRed-Express
UseAAVTagsNLSExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I11 PhiC31-NLS GG1-GG4
Plasmid#195894PurposeLevel 0 PhiC31 inegrase NLS with GG1 GG4 Golden gate linkersDepositorInsertPhiC31 integrase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I12 PhiC31-NLS GG2-GG4
Plasmid#195895PurposeLevel 0 PhiC31 inegrase NLS with GG2 GG4 Golden gate linkersDepositorInsertPhiC31 integrase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-ArgSen (-)P2A (-)NLS
Plasmid#184690PurposeLentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLSDepositorInsertArgSen (-)P2A (-)NLS
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-ArgSen (-)P2A (-)NLS
Plasmid#184699PurposeLentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLSDepositorInsertArgSen (-)P2A (-)NLS
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a
Plasmid#182126PurposepET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteriaDepositorInsert1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
1N/2CxNLS-cMyc-NLP-cMyc AspCas12a
Plasmid#182121PurposepET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteriaDepositorInsert1N/2CxNLS-cMyc-NLP-cMyc AspCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a
Plasmid#182124PurposepET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a in bacteriaDepositorInsert1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICH47811-pAtAct2::Cas9-nls::tSlATPase
Plasmid#160221PurposeCas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverseDepositorInsertpAtACT2::Cas9-nls::tSlATPase
UseSynthetic Biology; Moclo toolkit level 1 acceptor…TagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-Cas9-nls-CDS1
Plasmid#160231PurposeCas9-nuclear localization, level 0 of MoClo Golden Gate position CDS1DepositorInsertCas9-nuclear localization signal gene sequence, Golden Gate MoClo position CDS1
UseLevel 0 of moclo golden gate position cds1TagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2
Plasmid#186413PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.DepositorInsertlacZ (lacZ E. coli)
UseGateway destination vectorTagsNuclear localization signalExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ubc-nls-pcp-10xSunTag (SRv1.1)
Plasmid#185798Purposepcp-10xSunTag component for SunRISER SRv1.1DepositorInsertpcp-10xSunTag
UseTagsFactorXa site, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
ubc-nls-pcp-12xSunTag (SRv1.2)
Plasmid#185799Purposepcp-12xSunTag component for SunRISER SRv1.2DepositorInsertpcp-12xSunTag
UseTagsFactorXa site, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-NLS-QPAS1-VP16
Plasmid#186187PurposeAAV vector packaging QPAS1-VP16, a component of optogenetic system for NIR light-controllable transcriptional activationDepositorInsertNLS-QPAS1-VP16
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH321-1-Tier1-PhCMV-dCas9-3xNLS
Plasmid#169595PurposeTier-1 vector encoding PhCMV-driven dCas9-3xNLS expression (PhCMV-dCas9-3xNLS-pA).DepositorInsertdead S.pyogenes Cas9
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKSB- PUb-NLSmTurqSV40 GGGG AAGA
Plasmid#174535PurposePUb-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsertPUb-mTurquoise BsaI cassette
UseTagsExpressionInsectMutationPromoterAedes aegypti PolyubiquitinAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL
Plasmid#172208PurposeAAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1S promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS-SID4X_2A_EGFP_W3SL
Plasmid#172205PurposeAAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1FL promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(NG)-Tet1FL-SID4X-NLS_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-dt/CBA-EGFP-NLS
Plasmid#163918PurposeContains bidirectional reporter genes dtomato and nuclear-targeting EGFP.DepositorInsertdtomato and EGFP
UseLentiviralTagsExpressionMammalianMutationAdded nuclear localization signal to EGFPPromoterAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCaggs-NLS-PAmCherry1-GSS-EGFP
Plasmid#158000PurposeExpression of nucleus localized PAmCherry1 and EGFP fusion proteinDepositorInsertNLS-PAmCherry1-GSS-EGFP
UseTagsExpressionMammalianMutationPromoterActin promoter with CMV enhancerAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mTurquoise-3XNLS-WPRE
Plasmid#159949Purposeoptical regulation of nuclear exportDepositorInsertmTurquoise-3XNLS
UseAAVTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-Ypet-3XNLS-WPRE
Plasmid#159950Purposenucleus markerDepositorInsertYpet-3XNLS
UseAAVTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13(+7A)-IDR-YFP-NLS
Plasmid#145278PurposeMammalian Expression of Nuclear HOXD13 (+7A) IDR-YFP with SV40 NLSDepositorInsertHOXD13(+7A)-IDR (HOXD13 Human)
UseTagsExpressionMammalianMutationA-repeat expandedPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only