We narrowed to 66,338 results for: nin

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Ub-R-GFP

Plasmid

#11939

Depositor

Nico Dantuma

Publication

Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

Insert

Ub-R-GFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Arginine at…

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pcDNA3-N-Flag-NLRP3

Plasmid

#75127

Purpose

Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

Depositor

Bruce Beutler

Publication

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pLX313-Renilla luciferase

Plasmid

#118016

Purpose

Constitutive expression of Renilla luciferase

Depositor

William Hahn, David Root

Publication

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Renilla luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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APEX2-GBP

Plasmid

#67651

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCMV/SB11

Plasmid

#26552

Depositor

Perry Hackett

Publication

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty

Expression

Bacterial

Mutation

Methionine 243 to Glutamine Threonine 136 to Arg…

Available since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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M38 TOP-dGFP

Plasmid

#17114

Depositor

Randall Moon

Insert

Tcf/Lef sites (Ctnnb1 )

Tags

GFP

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pT3-myr-AKT-HA

Plasmid

#31789

Depositor

Xin Chen

Publication

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Tags

HA and Myr

Expression

Mammalian

Available since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

45 Articles

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pDEST-periHisMBP

Plasmid

#11086

Depositor

David Waugh

Publication

Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Type

Empty backbone

Tags

6xHis, MBP, and MBP signal peptide

Expression

Bacterial

Promoter

Tac (lactose/IPTG inducible)

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET28-MBP-TEV

Plasmid

#69929

Purpose

bacterial expression of MBP-TEV n-terminal fusions

Depositor

Zita Balklava, Thomas Wassmer

Publication

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Has service

Cloning Grade DNA

Type

Empty backbone

Tags

MBP and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

Plasmid

#138150

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Publication

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR1_BsmBI

Use

Lentiviral

Mutation

DR1 A>T

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pCRCT

Plasmid

#60621

Purpose

Plasmid encoding iCas9, tracrRNA and crRNAs

Depositor

Huimin Zhao

Publication

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pGEM-3z/601

Plasmid

#26656

Depositor

Jonathan Widom

Publication

Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

Insert

Expression

Bacterial

Available since

Nov. 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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p6XHis-T7(P266L)

Plasmid

#174866

Purpose

Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

Depositor

Anna Pyle

Publication

Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

Insert

T7 RNA polymerase

Tags

6XHis-tag

Expression

Bacterial

Mutation

P266L

Promoter

lac promoter

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLV-EF1a-IRES-Neo

Plasmid

#85139

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Publication

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Has service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pCMV-PEmax-P2A-BSD

Plasmid

#174821

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-BSD

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLenti SpBsmBI sgRNA Hygro

Plasmid

#62205

Purpose

An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

Depositor

Rene Maehr

Publication

Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

Has service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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pDD104 (Peft-3::Cre)

Plasmid

#47551

Purpose

Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

Depositor

Bob Goldstein

Publication

Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

Insert

Cre Recombinase

Use

Cre/Lox

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

Plasmid

#154473

Purpose

Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

Depositor

Mikko Taipale

Publication

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

SFFV

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pGP-CMV-jGCaMP8m

Plasmid

#162372

Purpose

Mammalian expression of ultrafast protein calcium sensor

Depositor

GENIE Project

Publication

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8m

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

CMV

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEJS578_DD-dSpyCas9-mCherry-APEX2

Plasmid

#108570

Purpose

Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

Depositor

Erik Sontheimer

Publication

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Insert

dSpyCas9

Use

CRISPR and Lentiviral

Tags

APEX2, Destabilization domains, Flag, and mCherry…

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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