We narrowed to 65,738 results for: nin

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Active Wnt10B-V5

Plasmid

#43823

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT10B (WNT10B Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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MDH2

Plasmid

#38792

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

MDH2 (MDH2 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag RET

Plasmid

#20614

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

RET (RET Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

R982C

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
35S-GWY-VP16/pAM

Plasmid

#201232

Purpose

Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-GWY-VP16

Expression

Plant

Promoter

CaMV 35S

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-MCP-huADARE488QT490A

Plasmid

#159922

Purpose

Fusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5

Depositor

Fei Chen

Article

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

Human ADAR2 E488QT490A (ADARB1 Human)

Use

AAV

Promoter

EF1a

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pKS004 - pCAGGS-3XFLAG-CTCF-eGFP

Plasmid

#156438

Purpose

For transient expression of mouse CTCF tagged with eGFP

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse CTCF (Ctcf Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-EGFP:Ajuba

Plasmid

#108229

Purpose

Lentiviral vector for Tet-inducible Ajuba expression

Depositor

Kenneth Irvine

Article

Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

Insert

AJUBA (AJUBA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Tet/ON Minimal CMV promoter

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pDicAID_nCas9-PmCDA-2A-NptII_ETR

Plasmid

#91695

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-DEPTOR

Plasmid

#73390

Purpose

Expresses HA and YFP tagged DEPTOR

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

DEP Domain Containing MTOR-Interacting Protein (DEPTOR Human)

Tags

HA and YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pEntr-Nkx2-5flbio

Plasmid

#32969

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

Nkx2-5 (Nkx2-5 Mouse)

Use

Gateway Cloning

Tags

flag-bio

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pAd GFP HDAC5 WT

Plasmid

#32217

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Use

Adenoviral

Tags

GFP

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
CRISPR-StAR4GN (Lenti)

Plasmid

#222693

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

PGK without BsmBI cutsite

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag003-Talin rod-mCherry

Plasmid

#133567

Purpose

Expresses SpyTag003-Talin rod-mCherry in mammalian cells

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-Talin rod-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 IL6

Plasmid

#67061

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL6 (IL6 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2R2

Plasmid

#15806

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2R2 (UBE2R2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Plasmid

#221235

Purpose

Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Use

Lentiviral

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLX311 MCL1

Plasmid

#117729

Purpose

Open reading frame vector encoding MCL1

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

MCL1L (MCL1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-SypHer2-DAAO-NES

Plasmid

#119184

Purpose

Fusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.

Depositor

Thomas Michel

Article

Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

Insert

SypHer2-DAAO-NES

Use

AAV

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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rLAMP1-mCherry-EGFP-Tet-On

Plasmid

#109015

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged mLAMP1 (lysosome marker)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

rLAMP1

Use

Lentiviral

Tags

mcherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSHS207 - Bacterial expression plasmid for WT SpCas9

Plasmid

#101199

Purpose

Bacterial expression plasmid for WT SpCas9

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2017

Availability

Academic Institutions and Nonprofits only

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