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We narrowed to 19,248 results for: Tat

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Showing: 5721 - 5740 of 19248 results

  1. AAV-KrasG12A/sgKras/Cre

    Plasmid
    #99849
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99849-plasmid-map-sequence-id-193789

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  2. AAV-KrasG13C/sgKras/Cre

    Plasmid
    #99856
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99856-plasmid-map-sequence-id-193794

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  3. pCMV-ABE8.20m-eVRQR-P2A-EGFP (LLH569)

    Plasmid
    #242656
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.20 A-to-G base editor with neVRQR(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8.20m-VRQR-S55R-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.20 mutations in TadA, eVRQR mutations in SpC…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242656-plasmid-map-sequence-id-488970

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  7. pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)

    Plasmid
    #208289
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA; mutations in iSpyMac
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208289-plasmid-map-sequence-id-410849

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  8. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

    Plasmid
    #210710
    Purpose
    This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9 (STAT1, MRTFA Staphylococcus aureus, Synthetic, Human)
    Use
    AAV and CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A/N580A
    Promoter
    M11
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210710-plasmid-map-sequence-id-416264

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  10. pCC336

    Plasmid
    #110167
    Purpose
    miniMos vector expresses ERKKTR(S43E,T55E,T62E)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)
    Depositor
    Iva Greenwald
    Article
    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.
    Insert
    ERK-KTR(S43E,T55E,T62E)-mClover-T2A-mCherry-H2B
    Use
    Minimos transposon
    Tags
    mCherry and mClover
    Expression
    Worm
    Mutation
    Phospho-acceptor sites mutated: S43E, T55E, T62E.…
    Promoter
    lin-31 promoter/enhancer
    Available Since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110167-plasmid-map-sequence-id-238139

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  11. pCC335

    Plasmid
    #110166
    Purpose
    miniMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)
    Depositor
    Iva Greenwald
    Article
    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.
    Insert
    ERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B
    Use
    Minimos transposon
    Tags
    mCherry and mClover
    Expression
    Worm
    Mutation
    Phospho-acceptor sites mutated: S43A, T55A, T62A.…
    Promoter
    lin-31 promoter/enhancer
    Available Since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110166-plasmid-map-sequence-id-235342

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  12. MB 45t3 thsS(t3)R-sfGFP_mCherry

    Plasmid
    #232470
    Purpose
    Optimized thiosulfate sensor with fluorescent reporter (GFP), constitutive mCherry
    Depositor
    Mikhail Shapiro
    Article
    Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:
    Inserts
    thsS(t3)
    thsR
    PphsA342
    sfGFP
    AxeTxe
    mCherry
    Expression
    Bacterial
    Mutation
    Contains the following mutations K286Q, Q350H, I…
    Promoter
    J23100 (TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC), J23…
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    232470-plasmid-map-sequence-id-467797

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  13. gCH132 (crCD55-4_crB2M-1_crKIT-2_crKIT-3_crCD81-1)

    Plasmid
    #217341
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Article
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Promoter
    hU6
    Available Since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    217341-plasmid-map-sequence-id-426767

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  14. pMIG-hNFATc2/C(RIT)-FLAG-AVITEV

    Plasmid
    #74053
    Purpose
    retroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 Human)
    Use
    Retroviral
    Tags
    AVI-TEV and FLAG
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    pMSCV-LTRs
    Available Since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74053-plasmid-map-sequence-id-142273

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  18. GRP94-COMP-blac-flag-his

    Plasmid
    #111008
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    endoplasmin, putative (GRP94) (PFL1070c Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111008-plasmid-map-sequence-id-224562

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  19. pZE21/UBP1/ClpS_V65I

    Plasmid
    #98567
    Purpose
    Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon
    Depositor
    George Church
    Article
    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11
    Inserts
    Truncated ubiquitin cleavase, codon optimized
    Mutated ATP-dependent Clp protease adapter protein
    Expression
    Bacterial
    Mutation
    Contains the V65I mutation that increases discrim…
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98567-plasmid-map-sequence-id-203054

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Showing: 5721 - 5740 of 19248 results