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CL7-LbCas12aD156R
Plasmid#164659PurposeBacterial expression of mutated LbCas12aD156R using CL7/Im7DepositorInsertLbCas12a-D156R
UseTagsThioredoxin, CL7, SUMOExpressionBacterialMutationchanged Aspartic Acid 156 to ArgininePromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
SENP2M497A
Plasmid#61088Purposecleave the SUMO off when use PETSUMO vector for express fusion proteinDepositorInsertSENP2M497A catalytic domain (SENP2 Human)
UseTagsHisExpressionBacterialMutationM497A; Catalytic domain is residues 364-589PromoterT7Available sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry_eDHFR_SIM#1
Plasmid#164646Purposetag SIM with mCherry and eDHFRDepositorInsertSIM
UseCre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(Q66L)-RagC
Plasmid#99700PurposeBacterial expression of RagA(Q66L)-RagCDepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationL91PPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136463
Plasmid#149527PurposeBacterial expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4136454
Plasmid#149529PurposeBacterial expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 13, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Pediculus humanus corporis PINK1 (143 - 575)
Plasmid#110751PurposeBacterial expression of PhPINK1 (crystallography)DepositorInsertPhum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
UseTagsN-His6-SUMOExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC
Plasmid#99649PurposeBacterial expression of wildtype Rag GTPase dimerDepositorUseTags10x His tag, Poly arginine linker, and SUMOExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(Q120L)
Plasmid#99701PurposeBacterial expression of RagA-RagC(Q120L)DepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationQ120LPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
eSrtA-Δ24-ASN
Plasmid#221849PurposeExpresses delta 24 alpha synuclein as 6XHis-SUMO-eSrtA-LPNTG-Δ24-ASN fusion proteinDepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionBacterialMutationNoPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY1935
Plasmid#212315PurposeExpress ApmFnuc protein with sumotagDepositorInsertIsvmimi
UseTags6xHis-thrombin site-TwinStrepTag-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 AMSHLP 16-218
Plasmid#180609PurposeBacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83DepositorInsertAMSHLP residues 16-218
UseTagsHIS-SUMOExpressionBacterialMutationResidues 16-218PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
SNAP-Kindlin
Plasmid#186136PurposeFor expression of recombinant Snap-labeled kindlin in bacteriaDepositorInsertFERMT2 (FERMT2 Human)
UseTagsHis6-sumoExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCm N-term MBP SMT3 NikJC199U
Plasmid#174363PurposeExpression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, MBP, SMT3, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(R37P)-RagC
Plasmid#99706PurposeBacterial expression of RagA(R37P)-RagCDepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationR37PPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(L91P)
Plasmid#99707PurposeBacterial expression of RagA-RagC(L91P)DepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationL91PPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(K84T)
Plasmid#99708PurposeBacterial expression of RagA-RagC(K84T)DepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationK84TPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(T21N)-RagC
Plasmid#99650PurposeBacterial expression of RagA(T21N)-RagCDepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationT21NPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(S75N)
Plasmid#99699PurposeBacterial expression of RagA-RagC(S75N)DepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationS75NPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(Q66L)-RagC(Q120L)
Plasmid#99702PurposeBacterial expression of RagA(Q66L)-RagC(Q120L)DepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationQ120L and Q66LPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-hUba2-His
Plasmid#53117Purposeexpression of full length human Uba2 with Cterminal His-tag in E.coliDepositorInsertUBA2 (UBA2 Human)
UseTagsHis and T7 tagExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
PCV-Cas9
Plasmid#123643PurposeExpresses Cas9 with an N-terminal HUH-tag called PCV2DepositorInsertsUseTags(EAAAK)8 linker and His6 + SUMOExpressionBacterialMutation2-116PromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINS-USP21 (USP, aa 196-565)
Plasmid#61585PurposeExpresses human USP21 (USP domain) in E. coli.DepositorInsertUSP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
UseTagsHis6-SUMOExpressionBacterialMutationDeleted aa 1-195.PromoterT7Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINS-AREL1
Plasmid#66710PurposeExpresses AREL1 HECT domain in E. coliDepositorInsertKIAA0317 (AREL1 )
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas9-PCV
Plasmid#123644PurposeExpresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coliDepositorInsertsUseTags6xHis and SUMOExpressionBacterialMutation2-116PromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136458
Plasmid#149531PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 13, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
WDV
Plasmid#226983PurposeExpresses the HUH endonuclease WDV, used as a no ligand control for DNA tension sensor experimentsDepositorInsertWDV (repA )
UseTags6xHis and SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528-PPxY1-GS-WW3e
Plasmid#166705Purposeexpresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cellsDepositorInsertAMOT PPxY1 fused to NEDD4L WW3
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9
Plasmid#133909PurposeBacterial expression of His6-Ubc9DepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only
