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Plasmid#86602PurposeCustom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.DepositorInsertMTST5
UseInternal standard reverse transcriptionTagsExpressionMutationPromoterT7Available sinceSept. 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
MTST6
Plasmid#86603PurposeCustom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.DepositorInsertMTST6
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMVS-V1
Plasmid#183094PurposeBasic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicusDepositorInsertspME2001
thermostable neomycin phosphotransferase gene
UseBacterial propagation and conjugativeTagsExpressionMutationPromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ppst93-MCRaeo-ND
Plasmid#192771PurposeExpresses ND-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NC
Plasmid#192770PurposeExpresses NC-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NG mcrBGA
Plasmid#192769PurposeExpresses NG-tagged MCRaeoBGA in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NG mcrBCGA
Plasmid#192768PurposeExpresses NG-tagged MCRaeoBCGA in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NG mcrBDGA
Plasmid#192767PurposeExpresses NG-tagged MCRaeoBDGA in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRmar-NG
Plasmid#192766PurposeExpresses NG-tagged MCRmar in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-CA
Plasmid#192765PurposeExpresses CA-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NB
Plasmid#192764PurposeExpresses NB-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NG
Plasmid#192763PurposeExpresses NG-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAcBac1-Mb-sfGFP WT
Plasmid#197569PurposeExpression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP WT
Pyl-tRNA (4 copies)
UseTagsV5-His6ExpressionMammalianMutationPromoterCMV and U6/H1Available sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-sfGFP 150TAG
Plasmid#197570PurposeExpression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP 150TAG
Pyl-tRNA (4 copies)
UseTagsV5-His6ExpressionMammalianMutationN150TAGPromoterCMV and U6/H1Available sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBBi-RP CaDXMT
Plasmid#139165PurposeSB-transposon with constitutive bi-directional promoter. One side contains Coffee arabica dimethylxanthine methyl transferase (CaDXMT) ; the other side contains RFP and puromycin resistance genes.DepositorInsert3,7-dimethylxanthine N-methyltransferase 1 [Coffea arabica]
UseTransposonTagsExpressionMammalianMutationPromoterEF1αAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSBBi-RP CaMXMT
Plasmid#139172PurposeSB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica monomethylxanthine methyltransferase (CaMXMT); the other side contains RFP and puromycin resistance genes.DepositorInsertmonomethylxanthine methyltransferase 1 [Coffea arabica]
UseTransposonTagsExpressionMammalianMutationPromoterEF1αAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCfB13089
Plasmid#212676PurposeTEF promoterDepositorInsertTEF
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12706
Plasmid#212677PurposeEXP promoterDepositorInsertEXP
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12710
Plasmid#212680PurposeSyn8 terminatorDepositorInsertSyn8
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCfB12709
Plasmid#212681PurposeSynGuo terminatorDepositorInsertSynGuo
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only